SERPINA6 ELISA Kit (Mouse) : 96 Wells (OKEH00288)

Catalog No: OKEH00288

Size:96T

Mouse SERPINA6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology SERPINA6 ELISA Kit (Mouse) (OKEH00288) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse SERPINA6 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SERPINA6 and incubated. The SERPINA6 found in the sample or standards competes with the biotinylated SERPINA6 for limited binding sites on the immobilized anti-mouse SERPINA6 antibody. Excess unbound biotinylated SERPINA6 and sample or standard SERPINA6 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SERPINA6 captured in the well and inversely proportional to the amount of SERPINA6 which was contained in the sample or standard.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.9% (n = 20)
  Mean Inter-assay CV%: < 9.5% (n = 20)
• ELISA Kit Component:
  • Anti-SERPINA6 Microplate: 96 Wells (12 x 8 Well strips)
  • SERPINA6 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X SERPINA6-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.85 ng/mL
• Specificity: Natural and recombinant Mouse Corticosteroid-binding globulin
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Serpina6
• Gene Full Name: serine (or cysteine) peptidase inhibitor, clade A, member 6
• Alias Symbols: Cbg;corticosteroid-binding globulin;serine (or cysteine) proteinase inhibitor, clade A, member 6;serpin A6;transcortin.
• NCBI Gene Id: 12401
• Protein Name: Q06770
• Description of Target: Corticosteroid-binding globulin
• Uniprot ID: https://www.uniprot.org/uniprot/Q06770
• Protein Accession #: NP_031644.1
• Nucleotide Accession #: NM_007618.3
• Protein Size (# AA): 397
• Molecular Weight: 45 kDa