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Catalog No: OKEH03593
Size:96T
Price: $625.00
SKU
OKEH03593
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SERPINA3N ELISA Kit (Rat) (OKEH03593) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpina3n has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Serpina3n is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpina3n captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit Component
ComponentAmount
Serpina3n Microplate96 Wells (12 x 8 Well strips)
Serpina3n Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Serpina3n Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Rat Serine protease inhibitor A3N
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSerpina3n
Gene Full Nameserine (or cysteine) peptidase inhibitor, clade A, member 3N
Alias Symbolscontrapsin-like protease inhibitor 6;CPi-26;serine protease inhibitor 2c;serine protease inhibitor 3;serine protease inhibitor A3N;serpin A3N;serpina3n-like protein;Spi-2.2;SPI3;SPI-3;Spin2c;Spin3.
NCBI Gene Id24795
Protein NameP09006
Description of TargetSerine protease inhibitor A3N
Uniprot IDhttps://www.uniprot.org/uniprot/P09006
Protein Accession #NP_113719.1
Nucleotide Accession #NM_031531.1
Protein Size (# AA)418
Molecular Weight47 kDa
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