SERPINA3N ELISA Kit (Mouse) (OKEH03592)

Catalog No: OKEH03592

Size:96T

Mouse SERPINA3N ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology SERPINA3N ELISA Kit (Mouse) (OKEH03592) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpina3n has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Serpina3n is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpina3n captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 4.4% (n = 20)
  Mean Inter-assay CV%: <= 7.2% (n = 20)
• ELISA Kit Component:
  • Serpina3n Microplate: 96 Wells (12 x 8 Well strips)
  • Serpina3n Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Serpina3n Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Mouse Serine protease inhibitor A3N
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for SERPINA3N ELISA Kit (Mouse) (OKEH03592) on CiteAb

Target Info

• Gene Symbol: SERPINA3N
• Gene Full Name: serine (or cysteine) peptidase inhibitor, clade A, member 3N
• Alias Symbols: Spi, ant, Spi2-2, Spi2.2, Spi2/e, Spi2/eb.4
• NCBI Gene Id: 20716
• Protein Name: Q91WP6
• Description of Target: Serine protease inhibitor A3N
• Uniprot ID: https://www.uniprot.org/uniprot/Q91WP6
• Protein Accession #: NP_033278.2
• Nucleotide Accession #: NM_009252.2
• Protein Size (# AA): 418
• Molecular Weight: 47 kDa