Catalog No: OKEH02416
Size:96T
Price: $625.00
SKU
OKEH02416
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SERPINA2 ELISA Kit (Human) (OKEH02416) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINA2 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
SERPINA2 Microplate96 Wells (12 x 8 Well strips)
SERPINA2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SERPINA2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity38 pg/mL
SpecificityNatural and recombinant Human Putative alpha-1-antitrypsin-related protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSERPINA2
Gene Full Nameserpin family A member 2 (gene/pseudogene)
Alias SymbolsProtease inhibitor 1-like;Serpin A2.
NCBI Gene Id390502
Protein NameP20848
Description of TargetPutative alpha-1-antitrypsin-related protein
Uniprot IDhttps://www.uniprot.org/uniprot/P20848
Protein Accession #NP_006211.2
Nucleotide Accession #NM_006220.2
Protein Size (# AA)421
Molecular Weight48 kDa
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