Catalog No: OKCD02973
Size:96WELLS
Price: $625.00
SKU
OKCD02973
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for SERPINA12 ELISA Kit (Mouse) (OKCD02973) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 15.6-1,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 6.8 pg/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin). No significant cross-reactivity or interference between Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | Serpina12 |
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Gene Full Name | serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 |
Alias Symbols | 4632419J12Rik;serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12;serpin A12;vaspin;visceral adipose tissue-derived serine protease inhibitor;visceral adipose-specific SERPIN. |
NCBI Gene Id | 68054 |
Protein Name | Serpin A12 |
Description of Target | Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues. |
Uniprot ID | Q7TMF5 |
Protein Accession # | NP_080811.1 |
Nucleotide Accession # | NM_026535.2 |
Protein Size (# AA) | 413 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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