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Catalog No: OKCD02973
Size:96WELLS
Price: $625.00
SKU
OKCD02973
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Datasheets/ManualsPrintable datasheet for SERPINA12 ELISA Kit (Mouse) (OKCD02973)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)99-105%96-103%94-103%84-91%
EDTA Plasma (n=5)80-101%85-97%90-101%83-102%
Heparin Plasma (n=5)87-102%78-90%81-102%87-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-10498
EDTA Plasma (n=5)84-10490
Heparin Plasma(n=5)94-10298
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SERPINA12 Microplate96 Wells (12 x 8 Well strips)
SERPINA12 Lyophilized Standard2
100X Biotinylated SERPINA12 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity6.8 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin). No significant cross-reactivity or interference between Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSerpina12
Gene Full Nameserine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
Alias Symbols4632419J12Rik;serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12;serpin A12;vaspin;visceral adipose tissue-derived serine protease inhibitor;visceral adipose-specific SERPIN.
NCBI Gene Id68054
Protein NameSerpin A12
Description of TargetAdipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
Uniprot IDQ7TMF5
Protein Accession #NP_080811.1
Nucleotide Accession #NM_026535.2
Protein Size (# AA)413
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