SERPINA12 ELISA Kit (Human) (OKCD00253)

Catalog No: OKCD00253 (Formerly GWB-KBBDZ1)

Size:96WELLS

Human SERPINA12 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	86-96%	79-93%	98-105%	80-89%
  EDTA plasma(n=5)	93-102%	95-104%	97-105%	84-99%
  heparin plasma(n=5)	78-105%	78-103%	88-95%	82-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	102
  EDTA plasma(n=5)	95-104	101
  heparin plasma(n=5)	86-95	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SERPINA12 Microplate: 96 Wells (12 x 8 Well Strips)
  • SERPINA12 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SERPINA12 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.062 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin). No significant cross-reactivity or interference between Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SERPINA12
• Gene Full Name: serpin family A member 12
• Alias Symbols: OL-64;serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12;serpin A12;serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12;vaspin;visceral adipose tissue-derived serine protease inhibitor;visceral adipose-specific SERPIN.
• NCBI Gene Id: 145264
• Protein Name: Serpin A12
• Description of Target: Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
• Uniprot ID: Q8IW75
• Protein Accession #: NP_001291390
• Nucleotide Accession #: NM_001304461.1
• Protein Size (# AA): 414