SEMA3E ELISA Kit (Mouse) (OKCD02879)

Catalog No: OKCD02879

Size:96WELLS

Mouse SEMA3E ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-97%	91-102%	93-103%	80-97%
  EDTA Plasma (n=5)	89-98%	98-105%	79-97%	78-99%
  Heparin Plasma (n=5)	92-101%	82-89%	89-96%	79-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3E (SEMA3E). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3E (SEMA3E). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3E (SEMA3E), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Semaphorin 3E (SEMA3E) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-105	102
  EDTA Plasma (n=5)	84-97	90
  Heparin Plasma(n=5)	88-102	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3E (SEMA3E) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3E (SEMA3E) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SEMA3E Microplate: 96 Wells (12 x 8 Well strips)
  • SEMA3E Lyophilized Standard: 2
  • 100X Biotinylated SEMA3E Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 3E (SEMA3E). No significant cross-reactivity or interference between Semaphorin 3E (SEMA3E) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Sema3e
• Gene Full Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
• Alias Symbols: 6430702L12;AA408817;mKIAA0331;Sem;sema H;Semah;semaphorin-3E;semaphorin-H.
• NCBI Gene Id: 20349
• Protein Name: Semaphorin-3E
• Description of Target: Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation.
• Uniprot ID: P70275
• Protein Accession #: NP_035478.2
• Nucleotide Accession #: NM_011348.2
• Protein Size (# AA): 775