Catalog No: OKCD02878
Size:96 Wells
Price: $750.00
SKU
OKCD02878
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)93-103%83-91%78-97%86-98%
EDTA Plasma (n=5)87-101%83-104%93-103%86-104%
Heparin Plasma (n=5)83-92%85-96%96-103%85-99%
ELISA Kit PrincipleAviva Systems Biology SEMA3E ELISA Kit (Human) (OKCD02878) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SEMA3E has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SEMA3E is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SEMA3E captured in well.
ELISA Kit Range1.25-20 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-9484
EDTA Plasma (n=5)79-9085
Heparin Plasma (n=5)87-9993
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level SEMA3E was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level SEMA3E was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-SEMA3E Microplate96 Wells (12 x 8 Well strips)
SEMA3E Lyophilized Standard 2 x 20 ng
100X Biotinylated SEMA3E Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.51 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Semaphorin 3E (SEMA3E). No significant cross-reactivity or interference between Semaphorin 3E (SEMA3E) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSEMA3E
Gene Full NameSemaphorin 3E
Alias SymbolsSEMAH, coll-5, M-SEMAH, M-SemaK
NCBI Gene Id9723
Protein NameSemaphorin-3E
Description of TargetPlays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation.
Uniprot IDO15041
Protein Accession #NP_001171600.1
Nucleotide Accession #NM_001178129.1
Molecular Weight775 kDa
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