SEMA3A ELISA Kit (Rat) (OKCD02841)

Catalog No: OKCD02841

Size:96WELLS

Rat SEMA3A ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-101%	80-97%	86-101%	79-99%
  EDTA Plasma (n=5)	99-105%	89-101%	93-102%	78-103%
  Heparin Plasma (n=5)	98-105%	80-102%	97-105%	85-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3A (SEMA3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3A (SEMA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3A (SEMA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Semaphorin 3A (SEMA3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-101	96
  EDTA Plasma (n=5)	90-103	95
  Heparin Plasma(n=5)	78-99	82

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SEMA3A Microplate: 96 Wells (12 x 8 Well strips)
  • SEMA3A Lyophilized Standard: 2
  • 100X Biotinylated SEMA3A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 6.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 3A (SEMA3A). No significant cross-reactivity or interference between Semaphorin 3A (SEMA3A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Sema3a
• Gene Full Name: semaphorin 3A
• Alias Symbols: sema domain, immunoglobulin domain (Ig), short basic domain, sec;sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A;sema III;semaphorin III;semaphorin-3A.
• NCBI Gene Id: 29751
• Protein Name: Semaphorin-3A
• Description of Target: May be involved in guiding growing axons towards their targets by forming a molecular boundary that instructs axons to engage in the formation of specific nerve tracts. Binds to neuropilin. Involved in the development of the olfactory system and in neuronal control of puberty (By similarity).
• Uniprot ID: Q63548
• Protein Accession #: NP_059006.1
• Nucleotide Accession #: NM_017310.1
• Protein Size (# AA): 772