Catalog No: OKEH01609
Size:96T
Price: $650.00
SKU
OKEH01609
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH01609
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SEMA3A ELISA Kit (Human) (OKEH01609) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SEMA3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SEMA3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SEMA3A captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
SEMA3A Microplate96 Wells (12 x 8 Well strips)
SEMA3A Lyophilized Standard2
100X Biotinylated SEMA3A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.058 ng/mL
SpecificityNatural and recombinant Human Semaphorin-3A
Publications

Konjac ceramide (kCer) regulates keratinocyte migration by Sema3A-like repulsion mechanism. Biochem Biophys Rep. 17, 132-138 (2019). 30623117

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolSEMA3A
Gene Full Namesemaphorin 3A
Alias Symbolscoll-1;COLL1;collapsin 1;HH16;Hsema-I;Hsema-III;sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A;SEMA1;SEMAD;SEMAIII;SEMAL;semaphorin D;semaphorin III;semaphorin L;semaphorin-3A;SemD.
NCBI Gene Id10371
Protein NameQ14563
Description of TargetSemaphorin-3A
Uniprot IDhttps://www.uniprot.org/uniprot/Q14563
Protein Accession #NP_006071.1
Nucleotide Accession #NM_006080.2
Protein Size (# AA)771
Molecular Weight89 kDa
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