SELPLG ELISA Kit (Mouse) (OKCD00263)

Catalog No: OKCD00263 (Formerly GWB-KBBAJ6)

Size:96WELLS

Mouse Selplg ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	80-91%	94-105%	88-104%	82-102%
  EDTA plasma(n=5)	92-101%	88-98%	93-105%	89-103%
  heparin plasma(n=5)	80-90%	85-97%	79-104%	80-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-Selectin Glycoprotein Ligand 1 (PSGL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to P-Selectin Glycoprotein Ligand 1 (PSGL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain P-Selectin Glycoprotein Ligand 1 (PSGL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of P-Selectin Glycoprotein Ligand 1 (PSGL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	83-104	97
  EDTA plasma(n=5)	92-99	96
  heparin plasma(n=5)	80-91	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level P-Selectin Glycoprotein Ligand 1 (PSGL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level P-Selectin Glycoprotein Ligand 1 (PSGL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Selplg Microplate: 96 Wells (12 x 8 Well Strips)
  • Selplg Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Selplg Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.066 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of P-Selectin Glycoprotein Ligand 1 (PSGL1). No significant cross-reactivity or interference between P-Selectin Glycoprotein Ligand 1 (PSGL1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SELPLG
• Gene Full Name: selectin P ligand
• Alias Symbols: Selectin P ligand.
• NCBI Gene Id: 20345
• Protein Name: P-selectin glycoprotein ligand 1
• Description of Target: A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
• Uniprot ID: Q62170
• Protein Accession #: NP_033177
• Nucleotide Accession #: NM_009151.3
• Protein Size (# AA): 397