SEC61A1 Antibody (OAGA00509)

Catalog No: OAGA00509

Size:100 ul

Sample (30 ug of whole cell lysate)
A: K562
10% SDS PAGE
OAGA00509 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SEC61A1(OAGA00509) antibody at 1:500 dilution.

Immunofluorescence analysis of methanol-fixed A431, using SEC61A1(OAGA00509) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq]
  Positive Controls: K562
  Cellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 413 and 476 of SEC61A1
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: SEC61A1
• Gene Full Name: Protein transport protein Sec61 subunit alpha isoform 1
• Alias Symbols: HNFJ4, SEC61, ADTKD5, HSEC61, SEC61A
• NCBI Gene Id: 29927
• Protein Name: Protein transport protein Sec61 subunit alpha isoform 1
• Uniprot ID: P61619
• Protein Accession #: NP_037468.1
• Molecular Weight: 52 kDa