SDC1/Syndecan-1 ELISA Kit (Human) (OKBB00608)

Catalog No: OKBB00608

Size:96 Wells

Human SDC1/Syndecan-1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human Syndecan-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Syndecan-1 has been precoated onto 96-well plates. Standards(NS0, Q23-G254) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Syndecan-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Syndecan-1 amount of sample captured in plate.
• ELISA Kit Range: 312 pg/ml - 20,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1.23	3.25	5.87	1.32	4.05	6.42
  SD	0.048	0.15	0.299	0.07	0.235	0.417
  CV%	3.9	4.6	5.1	5.3	5.8	6.5

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human Syndecan-1 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human Syndecan-1 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human Syndecan-1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants and serum
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human Syndecan-1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q23-G254
• Description: For quantitative detection of human Syndecan-1 in cell culture supernatants and serum.

Target Info

• Gene Symbol: SDC1
• Gene Full Name: syndecan 1
• Alias Symbols: CD138, P18827, SDC1, SYND1, Synd-1, Synd1, syndecan1, Syndecan-1
• NCBI Gene Id: 6382
• Protein Name: Syndecan-1
• Description of Target: Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
• Uniprot ID: P18827
• Protein Accession #: NP_001006947.1
• Nucleotide Accession #: NM_001006946.1