| Product Number |
P100866_T100 |
| Product Page |
www.avivasysbio.com/srf-antibody-n-terminal-region-p100866-t100.html |
| Name |
SRF Antibody - N-terminal region (P100866_T100) |
| Protein Size (# AA) |
508 amino acids |
| Molecular Weight |
52kDa |
| NCBI Gene Id |
6722 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Serum response factor (c-fos serum response element-binding transcription factor) |
| Description |
|
| Alias Symbols |
MCM1 |
| Peptide Sequence |
Synthetic peptide located within the following region: ATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIM |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Doi,H., et al., (2005) Arterioscler. Thromb. Vasc. Biol. 25 (11), 2328-2334 |
| Description of Target |
SRF is a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). |
| Protein Interactions |
Camk2d; ZIC3; ELK1; SRFBP1; SRF; NFKB1; PRKDC; CIRBP; ALDH3A1; SUMO1; UBE2I; SHBG; TRIM63; KDM6A; MYOCD; MKL1; CNTN2; CSNK2A1; SMARCA4; PELP1; KAT5; HDAC4; EPC1; SMAD7; HDAC3; MKL2; ATF6; BARX2; MAPKAPK2; FHL3; GATA4; CAMK2A; HOPX; PYCARD; NKX2-3; MINPP1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-SRF (P100866_T100) antibody |
| Blocking Peptide |
For anti-SRF (P100866_T100) antibody is Catalog # AAP31232 (Previous Catalog # AAPP01977) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human SRF |
| Uniprot ID |
P11831 |
| Protein Name |
Serum response factor |
| Sample Type Confirmation |
SRF is supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_003122 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_003131 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
SRF |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Zebrafish |
| Application |
CHIP, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-SRF Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysateSRF is supported by BioGPS gene expression data to be expressed in Jurkat |
|
| Image 2 | HCT116
 | Chromatin Immunoprecipitation (ChIP) Using SRF antibody - N-terminal region (P100866_T100) and HCT116 Cells |
|
