Product Number |
P100621_T100 |
Product Page |
www.avivasysbio.com/smad3-antibody-n-terminal-region-p100621-t100.html |
Name |
SMAD3 Antibody - N-terminal region (P100621_T100) |
Protein Size (# AA) |
425 amino acids |
Molecular Weight |
48kDa |
NCBI Gene Id |
4088 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
SMAD family member 3 |
Description |
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Alias Symbols |
LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436 |
Peptide Sequence |
Synthetic peptide located within the following region: FTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAIT |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Cheng,P.L., et al., (2004) Oncogene 23 (47), 7821-7838 |
Description of Target |
SMAD3 is a member of Smad family proteins. SMAD family is known to be important cytoplasmic mediators of signals from the transforming growth factor-beta (TGF-beta) receptor serine/threonine kinases. |
Protein Interactions |
TEKT4; CCDC33; CPSF7; MEOX2; HDAC1; PHC2; WWP1; AES; BLZF1; UBC; TP53; SMAD4; BACH1; MAFK; FOXO3; NEDD8; TRIM33; WWP2; SMURF2; FOXM1; TRIM62; TP63; OTUB1; NEDD4L; BAG3; TGFB1I1; CSNK1D; RUNX1; HCVgp1; TSC2; FHL3; FHL2; FHL1; Melk; IRF7; RNF111; LCK; KLF5; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-SMAD3 (P100621_T100) antibody |
Blocking Peptide |
For anti-SMAD3 (P100621_T100) antibody is Catalog # AAP30955 (Previous Catalog # AAPP01679) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD3 |
Uniprot ID |
P84022 |
Protein Accession # |
NP_005893 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_005902 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
SMAD3 |
Predicted Species Reactivity |
Human, Mouse, Rat, Dog, Guinea Pig, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100% |
Image 1 | Human Liver
| WB Suggested Anti-SMAD3 Antibody Titration: 2.0ug/ml Positive Control: Human Liver |
|
Image 2 | Human pancreas
| Human Pancrease |
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Image 3 | Mouse Testis
| Host: Mouse Target Name: SMAD3 Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml |
|
Image 4 | Human Urinary Bladder
| Rabbit Anti-SMAD3 Antibody Catalog Number: P100621_T100 Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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