PROTEIN A PEROXIDASE Conjugated (OORA00142)

Data Sheet
 
Product Number OORA00142
Product Page www.avivasysbio.com/protein-a-peroxidase-conjugated-oora00142.html
Name PROTEIN A PEROXIDASE Conjugated (OORA00142)
Molecular Weight 40000
Conjugation HRP
Concentration 2 mg/ml
Alias Symbols Staphylococcal protein A.
Product Format Lyophilized
Description of Target Immunoglobulin G-binding protein A
Reconstitution and Storage Restore with 1.0 mL of deionized water (or equivalent). Store vial at 4°C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Datasheets/Manuals Printable datasheet for OORA00142
Specificity Protein A Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.
Application Info Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate.
All assays should be optimized by the user. Recommcended dilutions may be listed below.
The suggested dilutions are: ELISA: 1:20,000 - 1:200,000 WB: 1:10,000 - 1:40,000 IHC: 1:1,000 - 1:5,000
Uniprot ID https://www.uniprot.org/uniprot/P38507
Protein Name P38507
Purification Protein A Peroxidase Conjugated was prepared from chromatographically pure Protein A.
Predicted Species Reactivity Staphylococcus aureus
Application Dot blot, Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
 

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