Product Number |
OKRA00052 |
Product Page |
www.avivasysbio.com/rapid-monoclonal-isotyping-kit-okra00052.html |
Name |
Rapid Monoclonal Isotyping Kit (OKRA00052) |
Purity |
Aviva's IsoMax Rapid Mouse Monoclonal Isotyping Kit is a rapid (5 minute) lateral flow assay with sensitivity equal to ELISA for monoclonal antibody class and subclass determination. The assay is designed to test antibody present in tissue culture superna |
Concentration |
Components in this kit are sufficient to run 10 isotype tests. |
Alias Symbols |
Isotyping kit, mouse subclass determination, IgG isotype, immunoglobulin isotyping |
Reconstitution and Storage |
This kit is stable for at least 18 months when stored at +20 C. |
Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
Additional Information |
Label: Unconjugated |
Application Info |
Each kit contains 10 packs of 2 cassettes each: one cassette for detecting IgG1, IgG2a and IgG2b; the other cassette for detecting IgG3, IgA and IgM. When a properly diluted sample containing a specific isotype antibody is added to the sample well, a specific class and subclass soluble complex is formed with the embedded gold conjugates. These complexes travel the length of the membrane and are resolved on the anti-isotype and anti-class-specific antibody impregnated membrane. A control line will appear on the membrane in the region marked "C" on the cassette indicating a successful run (positive internal control). Typically when antibodies are applied at a concentration of 10 ng/mL the best results are obtained by reading the cassette 5 to 10 minutes after application. Results should NOT be read after 10 minutes. |
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