Rapid Monoclonal Isotyping Kit (OKRA00052)

Data Sheet
 
Product Number OKRA00052
Product Page www.avivasysbio.com/rapid-monoclonal-isotyping-kit-okra00052.html
Name Rapid Monoclonal Isotyping Kit (OKRA00052)
Purity Aviva's IsoMax Rapid Mouse Monoclonal Isotyping Kit is a rapid (5 minute) lateral flow assay with sensitivity equal to ELISA for monoclonal antibody class and subclass determination. The assay is designed to test antibody present in tissue culture superna
Concentration Components in this kit are sufficient to run 10 isotype tests.
Alias Symbols Isotyping kit, mouse subclass determination, IgG isotype, immunoglobulin isotyping
Reconstitution and Storage This kit is stable for at least 18 months when stored at +20 C.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Additional Information Label: Unconjugated
Application Info Each kit contains 10 packs of 2 cassettes each: one cassette for detecting IgG1, IgG2a and IgG2b; the other cassette for detecting IgG3, IgA and IgM. When a properly diluted sample containing a specific isotype antibody is added to the sample well, a specific class and subclass soluble complex is formed with the embedded gold conjugates. These complexes travel the length of the membrane and are resolved on the anti-isotype and anti-class-specific antibody impregnated membrane. A control line will appear on the membrane in the region marked "C" on the cassette indicating a successful run (positive internal control). Typically when antibodies are applied at a concentration of 10 ng/mL the best results are obtained by reading the cassette 5 to 10 minutes after application. Results should NOT be read after 10 minutes.
 

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