Product Number |
OAPB00724 |
Product Page |
www.avivasysbio.com/med28-antibody-oapb00724.html |
Name |
MED28 Antibody (OAPB00724) |
Conjugation |
Unconjugated |
NCBI Gene Id |
80306 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Gene Full Name |
mediator complex subunit 28 |
Alias Symbols |
1500003D12Rik;EG1;endothelial-derived gene 1;endothelial-derived protein 1;magicin;Mediator complex subunit 28;mediator of RNA polymerase II transcription subunit 28;mediator of RNA polymerase II transcription, subunit 28 homolog;merlin and Grb2-interacting cytoskeletal protein;tumor angiogenesis marker EG-1. |
Product Format |
Liquid. MED28 Antibody is supplied in PBS containing 0.02% sodium azide. |
Reference |
Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell2004; 14:685-91.;Beyer KS, Beauchamp RL, Lee MF, et al. Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation. J. Biol. Chem.2007; 282:32152-7.;Wiederhold T, Lee MF, James M, et al. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene2004; 23:8815-25.;Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6. |
Description of Target |
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. |
Reconstitution and Storage |
MED28 antibody can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Datasheets/Manuals |
Printable datasheet for MED28 Antibody (OAPB00724) (OAPB00724) |
Additional Information |
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Application Info |
MED28 antibody can be used for detection of MED28 by Western blot at 1 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ?g/mL. For immunofluorescence start at 20 ?g/mL.
POSITIVE CONTROL: 1) Cat. No. OCPB00143 - Human Brain Tissue Lysate |
Blocking Peptide |
For anti-MED28 antibody is Catalog # OEPB02841 |
Immunogen |
MED28 antibody was raised against a 17 amino acid synthetic peptide from near the center of human MED28. The immunogen is located within amino acids 50 - 100 of MED28. |
Uniprot ID |
Q9H204 |
Protein Name |
Mediator of RNA polymerase II transcription subunit 28|mediator of RNA polymerase II transcription subunit 28 [Homo sapiens] |
Protein Accession # |
https://www.ncbi.nlm.nih.gov/protein/NP_079481 |
Purification |
Affinity Purified |
Gene Symbol |
MED28 |
Predicted Species Reactivity |
Bovine|Human|Mouse|Rat |
Application |
Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot |
Predicted Homology Based on Immunogen Sequence |
Human; Mouse |
Image 1 | Human brain
| Western blot analysis of MED28 in human brain tissue lysate with MED28 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. |
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Image 2 | Human brain
| Immunohistochemistry of MED28 in human brain tissue with MED28 antibody at 2.5 ug/mL. |
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