MED28 Antibody (OAPB00724)

Data Sheet
 
Product Number OAPB00724
Product Page www.avivasysbio.com/med28-antibody-oapb00724.html
Name MED28 Antibody (OAPB00724)
Conjugation Unconjugated
NCBI Gene Id 80306
Host Rabbit
Clonality Polyclonal
Concentration 1 mg/ml
Gene Full Name mediator complex subunit 28
Alias Symbols 1500003D12Rik;EG1;endothelial-derived gene 1;endothelial-derived protein 1;magicin;Mediator complex subunit 28;mediator of RNA polymerase II transcription subunit 28;mediator of RNA polymerase II transcription, subunit 28 homolog;merlin and Grb2-interacting cytoskeletal protein;tumor angiogenesis marker EG-1.
Product Format Liquid. MED28 Antibody is supplied in PBS containing 0.02% sodium azide.
Reference Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell2004; 14:685-91.;Beyer KS, Beauchamp RL, Lee MF, et al. Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation. J. Biol. Chem.2007; 282:32152-7.;Wiederhold T, Lee MF, James M, et al. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene2004; 23:8815-25.;Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.
Description of Target Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.
Reconstitution and Storage MED28 antibody can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Datasheets/Manuals Printable datasheet for MED28 Antibody (OAPB00724) (OAPB00724)
Additional Information Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Application Info MED28 antibody can be used for detection of MED28 by Western blot at 1 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ?g/mL. For immunofluorescence start at 20 ?g/mL.

POSITIVE CONTROL: 1) Cat. No. OCPB00143 - Human Brain Tissue Lysate
Blocking Peptide For anti-MED28 antibody is Catalog # OEPB02841
Immunogen MED28 antibody was raised against a 17 amino acid synthetic peptide from near the center of human MED28. The immunogen is located within amino acids 50 - 100 of MED28.
Uniprot ID Q9H204
Protein Name Mediator of RNA polymerase II transcription subunit 28|mediator of RNA polymerase II transcription subunit 28 [Homo sapiens]
Protein Accession # https://www.ncbi.nlm.nih.gov/protein/NP_079481
Purification Affinity Purified
Gene Symbol MED28
Predicted Species Reactivity Bovine|Human|Mouse|Rat
Application Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
Predicted Homology Based on Immunogen Sequence Human; Mouse
Image 1
Human brain
Western blot analysis of MED28 in human brain tissue lysate with MED28 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Image 2
Human brain
Immunohistochemistry of MED28 in human brain tissue with MED28 antibody at 2.5 ug/mL.
 

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