AKAP13 Antibody (OAAF02387)

• Product Number: OAAF02387
• Name: AKAP13 Antibody (OAAF02387)
• Molecular Weight: 307 kDa
• NCBI Gene Id: 11214
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: A-kinase anchoring protein 13
• Alias Symbols: A kinase (PRKA) anchor protein 13;AKAP-13;AKAP-Lbc;A-kinase anchor protein 13;ARHGEF13;breast cancer nuclear receptor-binding auxiliary protein;BRX;c-lbc;guanine nucleotide exchange factor Lbc;HA-3;Ht31;human thyroid-anchoring protein 31;LBC;LBC oncogene;lymphoid blast crisis oncogene;non-oncogenic Rho GTPase-specific GTP exchange factor;p47;PRKA13;protein kinase A-anchoring protein 13;PROTO-LB;PROTO-LBC.
• Product Format: Liquid
• Reference: Toksoz D., Oncogene 9:621-628(1994).
  Rubino D., Oncogene 16:2513-2526(1998).
  Sterpetti P., Mol. Cell. Biol. 19:1334-1345(1999).
• Description of Target: Scaffold protein that plays an important role in assembling signaling complexes downstream of several types of G protein-coupled receptors. Activates RHOA in response to signaling via G protein-coupled receptors via its function as Rho guanine nucleotide exchange factor (PubMed:11546812, PubMed:15229649, PubMed:23090968, PubMed:25186459, PubMed:24993829). May also activate other Rho family members (PubMed:11546812). Part of a kinase signaling complex that links ADRA1A and ADRA1B adrenergic receptor signaling to the activation of downstream p38 MAP kinases, such as MAPK11 and MAPK14 (PubMed:17537920, PubMed:23716597, PubMed:21224381). Part of a signaling complex that links ADRA1B signaling to the activation of RHOA and IKBKB/IKKB, leading to increased NF-kappa-B transcriptional activity (PubMed:23090968). Part of a RHOA-dependent signaling cascade that mediates responses to lysophosphatidic acid (LPA), a signaling molecule that activates G-protein coupled receptors and potentiates transcriptional activation of the glucocorticoid receptor NR3C1 (PubMed:16469733). Part of a signaling cascade that stimulates MEF2C-dependent gene expression in response to lysophosphatidic acid (LPA) (By similarity). Part of a signaling pathway that activates MAPK11 and/or MAPK14 and leads to increased transcription activation of the estrogen receptors ESR1 and ESR2 (PubMed:9627117, PubMed:11579095). Part of a signaling cascade that links cAMP and EGFR signaling to BRAF signaling and to PKA-mediated phosphorylation of KSR1, leading to the activation of downstream MAP kinases, such as MAPK1 or MAPK3 (PubMed:21102438). Functions as scaffold protein that anchors cAMP-dependent protein kinase (PKA) and PRKD1. This promotes activation of PRKD1, leading to increased phosphorylation of HDAC5 and ultimately cardiomyocyte hypertrophy (By similarity). Has no guanine nucleotide exchange activity on CDC42, Ras or Rac (PubMed:11546812). Required for normal embryonic heart development, and in particular for normal sarcomere formation in the developing cardiomyocytes (By similarity). Plays a role in cardiomyocyte growth and cardiac hypertrophy in response to activation of the beta-adrenergic receptor by phenylephrine or isoproterenol (PubMed:17537920, PubMed:23090968). Required for normal adaptive cardiac hypertrophy in response to pressure overload (PubMed:23716597). Plays a role in osteogenesis (By similarity).
• Reconstitution and Storage: -20°C
• Specificity: AKAP13 antibody detects endogenous levels of total AKAP13 protein.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from human AKAP13.
• Dilution: The optimal working dilution should be determined by the end user.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Uniprot ID: Q12802
• Protein Name: A-kinase anchor protein 13
• Protein Accession #: NP_001257475.1
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Gene Symbol: AKAP13
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: human heart tissue
  
  Immunohistochemistry analysis of paraffin-embedded human heart tissue, using AKAP13 Antibody. The picture on the right is treated with the synthesized peptide.
• Image 2: Jurkat/A549 cells
  
  Western blot analysis of extracts from Jurkat/A549 cells, using AKAP13 Antibody. The lane on the right is treated with the synthesized peptide.