| Product Number |
AVARP20016_T100 |
| Product Page |
www.avivasysbio.com/mmp2-antibody-c-terminal-region-avarp20016-t100.html |
| Name |
MMP2 Antibody - C-terminal region (AVARP20016_T100) |
| Protein Size (# AA) |
660 amino acids |
| Molecular Weight |
74kDa |
| NCBI Gene Id |
4313 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) |
| Description |
|
| Alias Symbols |
CLG4, MONA, CLG4A, MMP-2, TBE-1, MMP-II |
| Peptide Sequence |
Synthetic peptide located within the following region: AWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWL |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Collier, I.E., et al., (1988) J. Biol. Chem. 263:6579-6587. |
| Description of Target |
MMP-2 is involved in the cleavage of gelatin type I and collagen types IV, V, VII, X. |
| Protein Interactions |
HSP90AA1; CAND1; ITGB1; ITGAV; USP12; OSBPL10; SPOCK1; BACE1; PZP; MMP25; CLDN1; KISS1; MMP17; TIMP4; EPHB2; CXCL12; MMP14; BCAN; LCN2; TIMP1; TGFB1; TIMP3; TIMP2; THBS2; THBS1; CCL7; ACAN; LGALS3; IL1B; IGFBP3; FGFR1; DCN; COL18A1; COL6A3; COL1A1; COL7A1 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-MMP2 (AVARP20016_T100) antibody |
| Blocking Peptide |
For anti-MMP2 (AVARP20016_T100) antibody is Catalog # AAP30579 (Previous Catalog # AAPP01231) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human MMP2 |
| Uniprot ID |
P08253 |
| Protein Name |
72 kDa type IV collagenase |
| Publications |
Developmental Programming: Gestational Exposure to Excess Testosterone Alters Expression of Ovarian Matrix Metalloproteases and Their Target Proteins. Reprod Sci. , 1933719117697127 (2017). 28299992
TNF-a promotes breast cancer cell migration and enhances the concentration of membrane-associated proteases in lipid rafts. Cell Oncol (Dordr). 39, 353-63 (2016). 27042827 |
| Protein Accession # |
NP_004521 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_004530 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
MMP2 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Sheep: 100% |
| Image 1 | Human ovarian carcinoma
 | MMP1 in connective tissue in ovarian carcinoma was detected using HRP/DAB brown color stain.
Recommended for IHC on human tissue.
Working dilution 2-10 ug/mL |
|
| Image 2 | Human
 | Lanes:
Lane 1: 15ug MDA-MB-231 lysate
Lane 2: 15ug MCF7 lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
MMP2
Submitted by:
Katarzyna Augoff, University of Wroclaw
|
|
| Image 3 | Human Urinary Bladder
 | Rabbit Anti-MMP2 Antibody
Catalog Number: AVARP20016_T100
Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
| Image 4 | Human Lung
 | Host: Rabbit
Target Name: MMP2
Sample Type: Human Lung
Isoform 3: 66kDa
Isoform 2: 69kDa
Antibody Dilution: 1.0ug/ml |
|
| Image 5 | Human HCT116 Whole Cell
 | Host: Rabbit
Target Name: MMP2
Sample Tissue: Human HCT116 Whole Cell
Antibody Dilution: 1ug/ml |
|
