CTNNB1 Antibody - C-terminal region (AVARP14001_T100)

• Product Number: AVARP14001_T100
• Name: CTNNB1 Antibody - C-terminal region (AVARP14001_T100)
• Protein Size (# AA): 694 amino acids
• Molecular Weight: 86 kDa
• NCBI Gene Id: 1499
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Catenin (cadherin-associated protein), beta 1, 88kDa
• Alias Symbols: EVR7, CTNNB, MRD19, NEDSDV, armadillo
• Peptide Sequence: Synthetic peptide located within the following region: DPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Onilude OE,.(2006) Cancer Genet Cytogenet. Jul 15;168(2):158-61.
• Description of Target: CTNNB1 is involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
• Protein Interactions: PPARD; PLA2G4A; FBXO45; BTRC; SUMO1; UBC; HUWE1; CDH5; CDH2; TCF4; TBL1X; SOX1; SKP1; RNF220; CACYBP; FBXW11; SUMO2; SIAH1; Apc2; COPS5; RNF14; CDH1; SP1; KCTD1; MDM2; UBE2B; GSK3B; NEK2; RBX1; PTGS2; STRN3; LATS2; APC; ELAVL1; AMOT; ABL1; AXIN1; CBL; LEF
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-CTNNB1 (AVARP14001_T100) antibody is Catalog # AAP30238 (Previous Catalog # AAPS00103)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CTNNB1
• Sample Type Confirmation:

  CTNNB1 is supported by BioGPS gene expression data to be expressed in HCT116

• Protein Accession #: XP_001133664
• Purification: Protein A purified
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: CTNNB1
• Predicted Species Reactivity: Human, Mouse, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB, IP
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Zebrafish: 100%
• Image 1: Human Ovary
  
  Rabbit Anti-CTNNB1 Antibody
  Catalog Number: AVARP14001_T100
  Formalin Fixed Paraffin Embedded Tissue: Human Ovary Tissue
  Observed Staining: Cytoplasm, Plasma membrane
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 2: Mouse Liver
  
  Host: Rabbit
  Target Name: CTNNB1
  Sample Tissue: Mouse Liver
  Antibody Dilution: 1ug/ml
• Image 3: Mouse Skeletal Muscle
  
  Host: Rabbit
  Target Name: CTNNB1
  Sample Tissue: Mouse Skeletal Muscle
  Antibody Dilution: 1ug/ml
• Image 4: HEK293 Whole Cell Lysate
  
  CTNNB1 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with AVARP14001_T100 with 1:200 dilution. Western blot was performed using AVARP14001_T100 at 1/1000 dilution.
  Lane 1: Control IP in HEK293 Whole Cell Lysate.
  Lane 2: CTNNB1 IP with AVARP14001_T100 in HEK293 Whole Cell Lysate.
  Lane 3: Input of HEK293 Whole Cell Lysate.
• Image 5: Human Intestine
  
  Human Intestine
• Image 6: Human Heart
  
  Rabbit Anti-CTNNB1 Antibody
  Catalog Number: AVARP14001
  Paraffin Embedded Tissue: Human cardiac cell
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 7: Human MCF7
  
  Host: Rabbit
  Target Name: CTNNB1
  Sample Tissue: Human MCF7
  Antibody Dilution: 1.0ug/ml