Product Number |
AVARP13041_P050 |
Product Page |
www.avivasysbio.com/htr1a-antibody-n-terminal-region-avarp13041-p050.html |
Name |
HTR1A Antibody - N-terminal region (AVARP13041_P050) |
Protein Size (# AA) |
422 amino acids |
Molecular Weight |
46kDa |
NCBI Gene Id |
3350 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled |
Description |
|
Alias Symbols |
G-21, 5HT1a, PFMCD, 5-HT1A, 5-HT-1A, ADRBRL1, ADRB2RL1 |
Peptide Sequence |
Synthetic peptide located within the following region: GQGNNTTSPPAPFETGGNTTGISDVTVSYQVITSLLLGTLIFCAVLGNAC |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Czesak,M., (2006) J. Neurosci. 26 (6), 1864-1871 |
Description of Target |
This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibits adenylate cyclase activity. |
Protein Interactions |
UBC; RHOA; GABBR2; GPR26; S1PR1; S1PR3; HTR1D; HTR1B; GNAI3; HTR1A; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-HTR1A (AVARP13041_P050) antibody |
Blocking Peptide |
For anti-HTR1A (AVARP13041_P050) antibody is Catalog # AAP30710 |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human HTR1A |
Uniprot ID |
P08908 |
Protein Name |
5-hydroxytryptamine receptor 1A |
Publications |
Adeosun, S. O., Albert, P. R., Austin, M. C. & Iyo, A. H. 17beta-estradiol-induced regulation of the novel 5-HT1A-related transcription factors NUDR and Freud-1 in SH SY5Y cells. Cell. Mol. Neurobiol. 32, 517-21 (2012). 22328058
Cordeaux, Y., Pasupathy, D., Bacon, J., Charnock-Jones, D. S. & Smith, G. C. S. Characterization of serotonin receptors in pregnant human myometrium. J. Pharmacol. Exp. Ther. 328, 682-91 (2009). 19075042
Iyo, A. H. et al. Differential regulation of the serotonin 1 A transcriptional modulators five prime repressor element under dual repression-1 and nuclear-deformed epidermal autoregulatory factor by chronic stress. Neuroscience 163, 1119-27 (2009). 19647046
Szewczyk, B. et al. Decreased expression of Freud-1/CC2D1A, a transcriptional repressor of the 5-HT1A receptor, in the prefrontal cortex of subjects with major depression. Int. J. Neuropsychopharmacol. 13, 1089-101 (2010). 20392296
Szewczyk, B. et al. Gender-specific decrease in NUDR and 5-HT1A receptor proteins in the prefrontal cortex of subjects with major depressive disorder. Int. J. Neuropsychopharmacol. 12, 155-68 (2009). 18561871 |
Protein Accession # |
NP_000515 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_000524 |
Tested Species Reactivity |
Human |
Gene Symbol |
HTR1A |
Predicted Species Reactivity |
Human |
Application |
WB, IF |
Predicted Homology Based on Immunogen Sequence |
Human: 100% |
Image 1 | Human myometrial
| HTR1A antibody - N-terminal region (AVARP13041_P050) validated by WB using primary cultured neurons |
|
Image 2 | Human Jurkat
| Host: Rabbit Target Name: HTR1A Sample Type: Jurkat Whole Cell lysates Antibody Dilution: 1.0ug/ml |
|
Image 3 | Jurkat
| Host: Rabbit Target Name: HTR1A Sample Type: Jurkat Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1.0ug/mL Peptide Concentration: 1.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12% |
|
Image 4 | Primary cultured neurons 5HT1A Receptors
| Primary cultured neurons 5HT1A Receptors
Dilution:
Primary: 1:200
Secondary: 1:2000 |
|