Product Number |
AVARP09051_P050 |
Product Page |
www.avivasysbio.com/srebf1-antibody-middle-region-avarp09051-p050.html |
Name |
SREBF1 Antibody - middle region (AVARP09051_P050) |
Protein Size (# AA) |
1177 amino acids |
Molecular Weight |
125kDa |
NCBI Gene Id |
6720 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Sterol regulatory element binding transcription factor 1 |
Alias Symbols |
HMD, IFAP2, SREBP1, bHLHd1 |
Peptide Sequence |
Synthetic peptide located within the following region: DAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSR |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Motoyama,K., (2006) Biochem. Biophys. Res. Commun. 342 (1), 354-360 |
Description of Target |
SREBF1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family.This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. |
Protein Interactions |
UBC; ERLIN2; FBXW7; SMARCD3; HSP90AA1; SUMO1; ATXN1L; INSIG2; SCAP; ATXN1; LMNA; INSIG1; EGF; SREBF2; SREBF1; ZBTB7A; ZBTB7C; SUMO2; MED6; MED13; MED12; MED24; MED26; MED17; MED23; MED14; USF1; MED1; GSK3B; CREBBP; CDK8; MED25; MED15; MED7; MED21; SIRT1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-SREBF1 (AVARP09051_P050) antibody |
Blocking Peptide |
For anti-SREBF1 (AVARP09051_P050) antibody is Catalog # AAP30613 (Previous Catalog # AAPP01266) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human SREBF1 |
Uniprot ID |
P36956 |
Protein Name |
Sterol regulatory element-binding protein 1 |
Protein Accession # |
NP_001005291 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_001005291 |
Tested Species Reactivity |
Human |
Gene Symbol |
SREBF1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Goat, Horse |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 88%; Goat: 88%; Horse: 80%; Human: 82%; Mouse: 93%; Rat: 100% |
Image 1 | Human 293T
| WB Suggested Anti-SREBF1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Transfected 293T |
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Image 2 | |
Image 3 | Human Liver
| Human Liver |
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Image 4 | Human liver tissue
| Rabbit Anti-SREBF1 Antibody Catalog Number: AVARP09051_P050 Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue Observed Staining: Nucleus in hepatocytes Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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