SREBF1 Antibody - middle region (AVARP09051_P050)

Data Sheet
 
Product Number AVARP09051_P050
Product Page www.avivasysbio.com/srebf1-antibody-middle-region-avarp09051-p050.html
Name SREBF1 Antibody - middle region (AVARP09051_P050)
Protein Size (# AA) 1177 amino acids
Molecular Weight 125kDa
NCBI Gene Id 6720
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Sterol regulatory element binding transcription factor 1
Alias Symbols HMD, IFAP2, SREBP1, bHLHd1
Peptide Sequence Synthetic peptide located within the following region: DAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSR
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Motoyama,K., (2006) Biochem. Biophys. Res. Commun. 342 (1), 354-360
Description of Target SREBF1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family.This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene.
Protein Interactions UBC; ERLIN2; FBXW7; SMARCD3; HSP90AA1; SUMO1; ATXN1L; INSIG2; SCAP; ATXN1; LMNA; INSIG1; EGF; SREBF2; SREBF1; ZBTB7A; ZBTB7C; SUMO2; MED6; MED13; MED12; MED24; MED26; MED17; MED23; MED14; USF1; MED1; GSK3B; CREBBP; CDK8; MED25; MED15; MED7; MED21; SIRT1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-SREBF1 (AVARP09051_P050) antibody
Blocking Peptide For anti-SREBF1 (AVARP09051_P050) antibody is Catalog # AAP30613 (Previous Catalog # AAPP01266)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human SREBF1
Uniprot ID P36956
Protein Name Sterol regulatory element-binding protein 1
Protein Accession # NP_001005291
Purification Affinity Purified
Nucleotide Accession # NM_001005291
Tested Species Reactivity Human
Gene Symbol SREBF1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Goat, Horse
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 88%; Goat: 88%; Horse: 80%; Human: 82%; Mouse: 93%; Rat: 100%
Image 1
Human 293T
WB Suggested Anti-SREBF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Transfected 293T
Image 2

Image 3
Human Liver
Human Liver
Image 4
Human liver tissue
Rabbit Anti-SREBF1 Antibody
Catalog Number: AVARP09051_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Nucleus in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com