PDCD4 Antibody - N-terminal region (AVARP09045_P050)

• Product Number: AVARP09045_P050
• Name: PDCD4 Antibody - N-terminal region (AVARP09045_P050)
• Protein Size (# AA): 458 amino acids
• Molecular Weight: 50kDa
• NCBI Gene Id: 27250
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Programmed cell death 4 (neoplastic transformation inhibitor)
• Alias Symbols: H731
• Peptide Sequence: Synthetic peptide located within the following region: MTKYPDNLSDSLFSGDEENAGTEEIKNEINGNWISASSINEARINAKAKR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Yang,H.S., et al., (2004) Mol. Cell. Biol. 24 (9), 3894-3906
• Description of Target: PDCD4 is a protein localized to the nucleus in proliferating cells. Expression of its gene is modulated by cytokines in natural killer and T cells. PDCD4 is thought to play a role in apoptosis but the specific role has not yet been determined.This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified.
• Protein Interactions: EIF4A2; FBXW11; EIF4A3; PAIP1; ARPC1B; UFD1L; TPD52; BTF3; AAMP; IGSF8; CD81; DAXX; UBC; CSNK2A1; VCP; NR1H2; PSEN2; PSEN1; NAP1L1; APOE; BTRC; RPS6KA1; EIF4A1; ELAVL1; SUMO2; EMD; PDCD4; EIF4G1; RPL5; RPLP1; RPS13;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-PDCD4 (AVARP09045_P050) antibody is Catalog # AAP30594 (Previous Catalog # AAPP01246)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PDCD4
• Uniprot ID: Q53EL6
• Protein Name: Programmed cell death protein 4
• Publications:

  Folini, M. et al. miR-21: an oncomir on strike in prostate cancer. Mol. Cancer 9, 12 (2010). 20092645

  Pan, Q., Luo, X. & Chegini, N. microRNA 21: response to hormonal therapies and regulatory function in leiomyoma, transformed leiomyoma and leiomyosarcoma cells. Mol. Hum. Reprod. 16, 215-27 (2010). 19906824

• Protein Accession #: NP_663314
• Purification: Affinity Purified
• Nucleotide Accession #: NM_145341
• Tested Species Reactivity: Human
• Gene Symbol: PDCD4
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: HepG2
  
  Host: Rabbit
  Target Name: PDCD4
  Sample Type: HepG2
  Lane A: Primary Antibody
  Lane B: Primary Antibody + Blocking Peptide
  Primary Antibody Concentration: 2.0ug/mL
  Peptide Concentration: 2.0ug/mL
  Lysate Quantity: 25ug/lane
  Gel Concentration: 12%
• Image 2: Human Jurkat
  
  WB Suggested Anti-PDCD4 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: Jurkat cell lysate