| Product Number |
AVARP03038_P050 |
| Product Page |
www.avivasysbio.com/ccnd3-antibody-c-terminal-region-avarp03038-p050.html |
| Name |
CCND3 Antibody - C-terminal region (AVARP03038_P050) |
| Protein Size (# AA) |
292 amino acids |
| Molecular Weight |
33kDa |
| NCBI Gene Id |
896 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Cyclin D3 |
| Description |
|
| Peptide Sequence |
Synthetic peptide located within the following region: CQEQIEAALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Pruneri,G., et al., (2004) Int. J. Cancer 112 (1), 71-77 |
| Description of Target |
CCND3 encodes a protein that belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. |
| Protein Interactions |
POLR3GL; TFIP11; CDKN1A; CDK6; CDK4; CDK5; MCM10; CDKN1B; CDK2; CDK11B; FAS; SIL1; EIF3K; ATF5; VDR; MAPK6; NME1; MYB; GC; ETV1; RBL2; POLD1; CDK14; APP; RB1; UBC; PPP1CC; PPP1CB; PPP1CA; NCOA2; MAF1; NPDC1; DMTF1; CRABP2; RARA; DTNBP1; AKAP8; EFEMP2; CAS |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-CCND3 (AVARP03038_P050) antibody |
| Blocking Peptide |
For anti-CCND3 (AVARP03038_P050) antibody is Catalog # AAP30245 (Previous Catalog # AAPP00415) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human CCND3 |
| Uniprot ID |
P30281 |
| Protein Name |
G1/S-specific cyclin-D3 |
| Publications |
Discovery of Human-Similar Gene Fusions in Canine Cancers. Cancer Res. 77, 5721-5727 (2017). 28883003 |
| Sample Type Confirmation |
CCND3 is supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_001751 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001760 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
CCND3 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Sheep |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 92%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Sheep: 100% |
| Image 1 | Human Muscle
 | Human Muscle |
|
| Image 2 | Human Lymph Node
 | Rabbit Anti-CCND3 Antibody
Catalog Number: AVARP03038_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:600
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
| Image 3 | Human Jurkat
 | WB Suggested Anti-CCND3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysateCCND3 is supported by BioGPS gene expression data to be expressed in Jurkat |
|
| Image 4 | Mouse Spleen
 | Host: Mouse
Target Name: CCND3
Sample Tissue: Mouse Spleen
Antibody Dilution: 1ug/ml |
|
