CASP3 Antibody - N-terminal region (AVARP00021_T100)

Data Sheet
 
Product Number AVARP00021_T100
Product Page www.avivasysbio.com/casp3-antibody-n-terminal-region-avarp00021-t100.html
Name CASP3 Antibody - N-terminal region (AVARP00021_T100)
Protein Size (# AA) 277 amino acids
Molecular Weight 32 kDa
NCBI Gene Id 836
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Caspase 3, apoptosis-related cysteine peptidase
Alias Symbols CPP32, SCA-1, CPP32B
Peptide Sequence Synthetic peptide located within the following region: MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIII
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Selvakumar,P., et al., (2006) FEBS Lett. 580 (8), 2021-2026
Description of Target CASP3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Protein Interactions BAX; PARP1; PTGES3; XIAP; CRYAB; BIRC7; UBC; MDM2; Gzmb; VIM; STAT1; SPTAN1; ROCK1; GSN; DFFA; CASP9; CASP7; CASP6; CASP2; BID; ARHGDIA; TNFSF10; PLA2G4B; CDKN1A; RB1; PRKDC; GLRX; GATA1; SOCS5; YWHAG; MAPK9; MAPK8; SP1; PKN1; CASP8; BIRC3; BIRC2; ZBTB16;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-CASP3 (AVARP00021_T100) antibody
Blocking Peptide For anti-CASP3 (AVARP00021_T100) antibody is Catalog # AAP30492 (Previous Catalog # AAPP01128)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CASP3
Uniprot ID P42574
Protein Name Caspase-3
Publications

A scaffold-free surface culture of B16F10 murine melanoma cells based on magnetic levitation. Cytotechnology. 68, 2323-2334 (2016). 27670438

Protein Accession # NP_004337
Purification Protein A purified
Nucleotide Accession # NM_004346
Tested Species Reactivity Human
Gene Symbol CASP3
Predicted Species Reactivity Human, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 84%; Dog: 84%; Guinea Pig: 84%; Horse: 92%; Human: 100%; Pig: 92%; Rabbit: 84%; Rat: 84%
Image 1
Human Liver
WB Suggested Anti-CASP3 Antibody Titration: 5ug/ml
Positive Control: Human Liver
Image 2
Hela
Immunofluorescent Caspase 3 detection in HeLa cells after induction of apoptosis (red fluorescence). Nuclei were stained with DAPI (blue fluorescence).
Image 3

 

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