Product Number |
AVARP00021_T100 |
Product Page |
www.avivasysbio.com/casp3-antibody-n-terminal-region-avarp00021-t100.html |
Name |
CASP3 Antibody - N-terminal region (AVARP00021_T100) |
Protein Size (# AA) |
277 amino acids |
Molecular Weight |
32 kDa |
NCBI Gene Id |
836 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Caspase 3, apoptosis-related cysteine peptidase |
Alias Symbols |
CPP32, SCA-1, CPP32B |
Peptide Sequence |
Synthetic peptide located within the following region: MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIII |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Selvakumar,P., et al., (2006) FEBS Lett. 580 (8), 2021-2026 |
Description of Target |
CASP3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. |
Protein Interactions |
BAX; PARP1; PTGES3; XIAP; CRYAB; BIRC7; UBC; MDM2; Gzmb; VIM; STAT1; SPTAN1; ROCK1; GSN; DFFA; CASP9; CASP7; CASP6; CASP2; BID; ARHGDIA; TNFSF10; PLA2G4B; CDKN1A; RB1; PRKDC; GLRX; GATA1; SOCS5; YWHAG; MAPK9; MAPK8; SP1; PKN1; CASP8; BIRC3; BIRC2; ZBTB16; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-CASP3 (AVARP00021_T100) antibody |
Blocking Peptide |
For anti-CASP3 (AVARP00021_T100) antibody is Catalog # AAP30492 (Previous Catalog # AAPP01128) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human CASP3 |
Uniprot ID |
P42574 |
Protein Name |
Caspase-3 |
Publications |
A scaffold-free surface culture of B16F10 murine melanoma cells based on magnetic levitation. Cytotechnology. 68, 2323-2334 (2016). 27670438 |
Protein Accession # |
NP_004337 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_004346 |
Tested Species Reactivity |
Human |
Gene Symbol |
CASP3 |
Predicted Species Reactivity |
Human, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 84%; Dog: 84%; Guinea Pig: 84%; Horse: 92%; Human: 100%; Pig: 92%; Rabbit: 84%; Rat: 84% |
Image 1 | Human Liver
| WB Suggested Anti-CASP3 Antibody Titration: 5ug/ml Positive Control: Human Liver |
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Image 2 | Hela
| Immunofluorescent Caspase 3 detection in HeLa cells after induction of apoptosis (red fluorescence). Nuclei were stained with DAPI (blue fluorescence). |
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Image 3 | |