EIF3A Antibody - C-terminal region (ARP61702_P050)

• Product Number: ARP61702_P050
• Name: EIF3A Antibody - C-terminal region (ARP61702_P050)
• Protein Size (# AA): 1382 amino acids
• Molecular Weight: 152kDa
• Subunit: A
• NCBI Gene Id: 8661
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Eukaryotic translation initiation factor 3, subunit A
• Alias Symbols: EIF3, P167, p180, p185, TIF32, EIF3S10, eIF3-p170, eIF3-theta
• Peptide Sequence: Synthetic peptide located within the following region: PPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: EIF3A is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
• Protein Interactions: UBC; REL; TFIP11; WWP2; SUMO2; STAU1; LGR4; WWOX; EED; SHFM1; RNF2; EIF3CL; YTHDF2; LARP1; NUDT21; EIF3C; DYNC1I2; RPS26; RPS25; RPS23; RPS20; RPS18; RPS12; RPS10; RPS9; RPS3A; RPS3; FBXO6; TARDBP; EIF3D; UBD; ICAM1; CD81; RIOK2; IGSF8; BBS4; BBS1; gag-po
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-EIF3A (ARP61702_P050) antibody is Catalog # AAP61702 (Previous Catalog # AAPP47790)
• Uniprot ID: Q14152
• Protein Name: Eukaryotic translation initiation factor 3 subunit A
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that EIF3A is expressed in HeLa

• Protein Accession #: NP_003741
• Purification: Affinity Purified
• Nucleotide Accession #: NM_003750
• Tested Species Reactivity: Human
• Gene Symbol: EIF3A
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
• Image 1: Human HeLa
  
  WB Suggested Anti-EIF3A Antibody
  Titration: 1.0 ug/ml
  Positive Control: Hela Whole CellThere is BioGPS gene expression data showing that EIF3A is expressed in HeLa
• Image 2: Human Kidney
  
  Rabbit Anti-EIF3A antibody
  Catalog Number: ARP61702
  Formalin Fixed Paraffin Embedded Tissue: Human Kidney
  Primary antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20x
  Exposure Time: 0.5-2.0sec
• Image 3: Human Spleen
  
  Rabbit Anti-EIF3A antibody
  Catalog Number: ARP61702
  Formalin Fixed Paraffin Embedded Tissue: Human Spleen
  Primary antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20x
  Exposure Time: 0.5-2.0sec