Product Number |
ARP59608_P050 |
Product Page |
www.avivasysbio.com/manf-antibody-c-terminal-region-arp59608-p050.html |
Name |
MANF Antibody - C-terminal region (ARP59608_P050) |
Protein Size (# AA) |
185 amino acids |
Molecular Weight |
21kDa |
NCBI Gene Id |
7873 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Mesencephalic astrocyte-derived neurotrophic factor |
Alias Symbols |
ARP, ARMET |
Peptide Sequence |
Synthetic peptide located within the following region: EKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCK |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Description of Target |
MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of MANF gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region, including a specific mutation that changes the previously numbered codon 50 from ATG to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related. |
Protein Interactions |
UBC; MDM2; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-MANF (ARP59608_P050) antibody |
Blocking Peptide |
For anti-MANF (ARP59608_P050) antibody is Catalog # AAP59608 (Previous Catalog # AAPP46308) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human MANF |
Uniprot ID |
A8K878 |
Protein Name |
cDNA FLJ77177, highly similar to Homo sapiens arginine-rich, mutated in early stage tumors (ARMET), mRNA EMBL BAF84932.1 |
Sample Type Confirmation |
MANF is supported by BioGPS gene expression data to be expressed in HCT15 |
Protein Accession # |
NP_006001 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_006010 |
Tested Species Reactivity |
Human |
Gene Symbol |
MANF |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
Image 1 | Human HCT15
| WB Suggested Anti-MANF Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: HCT15 cell lysateMANF is supported by BioGPS gene expression data to be expressed in HCT15 |
|
|