HIST1H1E Antibody - N-terminal region (ARP58288_P050)

• Product Number: ARP58288_P050
• Name: HIST1H1E Antibody - N-terminal region (ARP58288_P050)
• Protein Size (# AA): 219 amino acids
• Molecular Weight: 22 kDa
• NCBI Gene Id: 3008
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Histone cluster 1, H1e
• Alias Symbols: H1E, H1.4, H1F4, RMNS, H1s-4, HIST1H1E, dJ221C16.5
• Peptide Sequence: Synthetic peptide located within the following region: MSETAPAAPAAPAPAEKTPVKKKARKSAGAAKRKASGPPVSELITKAVAA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
• Protein Interactions: UBC; HIST1H3A; RNF2; EXOSC4; TOM1; VPS26A; ICAM1; CD81; IGSF8; PAN2; ILF3; IL7R; MED8; CBX5; SIRT7; CBX3; SUMO2; IRAK4; PRKCA; HDGF; SUMO1; Mapk13; L3MBTL1; EHMT2; CBX1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-HIST1H1E (ARP58288_P050) antibody is Catalog # AAP58288 (Previous Catalog # AAPP33014)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HIST1H1E
• Uniprot ID: P10412
• Protein Name: Histone H1.4
• Protein Accession #: NP_005312
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005321
• Tested Species Reactivity: Human
• Gene Symbol: HIST1H1E
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 93%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 93%
• Image 1: Human ACHN
  
  WB Suggested Anti-HIST1H1E Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: ACHN cell lysate
• Image 2: THP-1, A549
  
  Host: Rabbit
  Target: HIST1H1E
  Positive control (+): THP-1 (N30)
  Negative control (-): A549 (N03)
  Antibody concentration: 3ug/ml