| Product Number |
ARP57828_P050 |
| Product Page |
www.avivasysbio.com/prkaca-antibody-n-terminal-region-arp57828-p050.html |
| Name |
PRKACA Antibody - N-terminal region (ARP57828_P050) |
| Protein Size (# AA) |
343 amino acids |
| Molecular Weight |
40 |
| NCBI Gene Id |
5566 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Protein kinase, cAMP-dependent, catalytic, alpha |
| Alias Symbols |
CAFD1, PKACA, PPNAD4 |
| Peptide Sequence |
Synthetic peptide located within the following region: MASNSSDVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRV |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Description of Target |
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Protein Interactions |
MAPT; UBC; PALM2-AKAP2; NASP; MARCKS; CALU; CALR; TRMT10A; ARPC5L; AARSD1; UGDH; TPD52; SH3GL1; RPA2; SH3GLB1; UBQLN2; ARPC1A; ARPC2; ARPC3; ARPC5; smo; CYP3A4; GNA13; DYRK1B; TBXA2R; ITGA2B; GSK3B; PDE4D; EGFR; CALD1; BAD; HSP90AB1; HSP90AA1; C2orf88; CD |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PRKACA (ARP57828_P050) antibody |
| Blocking Peptide |
For anti-PRKACA (ARP57828_P050) antibody is Catalog # AAP57828 (Previous Catalog # AAPP43102) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human PRKACA |
| Uniprot ID |
A8K8B9 |
| Protein Name |
cDNA FLJ77368, highly similar to Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 2, mRNA EMBL BAF84973.1 |
| Protein Accession # |
NP_997401 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_207518 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
PRKACA |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Sheep, Zebrafish |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 93%; Sheep: 100%; Zebrafish: 86% |
| Image 1 | Human Muscle
 | WB Suggested Anti-PRKACA Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Muscle |
|
| Image 2 | Human heart
 | Rabbit Anti-PRKACA Antibody
Catalog Number: ARP57828_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
