||PRKACA antibody - N-terminal region (ARP57828_P050)
||MGC102831, MGC48865, PKACA
|Protein Size (# AA)
||343 amino acids
||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
|NCBI Gene Id
||Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
|Official Gene Full Name
||Protein kinase, cAMP-dependent, catalytic, alpha
||This is a rabbit polyclonal antibody against PRKACA. It was validated on Western Blot using a cell lysate as a positive control. Aviva Systems Biology strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (firstname.lastname@example.org).
||Synthetic peptide located within the following region: MASNSSDVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRV
|Description of Target
||cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
||MAPT; UBC; PALM2-AKAP2; NASP; MARCKS; CALU; CALR; TRMT10A; ARPC5L; AARSD1; UGDH; TPD52; SH3GL1; RPA2; SH3GLB1; UBQLN2; ARPC1A; ARPC2; ARPC3; ARPC5; smo; CYP3A4; GNA13; DYRK1B; TBXA2R; ITGA2B; GSK3B; PDE4D; EGFR; CALD1; BAD; HSP90AB1; HSP90AA1; C2orf88; CD
|Reconstitution and Storage
||For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
||Domestic: within 1-2 days delivery International: 1-2 days
||For anti-PRKACA (ARP57828_P050) antibody is Catalog # AAP57828 (Previous Catalog # AAPP43102)
||The immunogen is a synthetic peptide directed towards the N terminal region of human PRKACA
|Complete computational species homology data
||Find tissues and cell lines supported by DNA array analysis to express PRKACA.
||cDNA FLJ77368, highly similar to Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 2, mRNA EMBL BAF84973.1
|Protein Accession #
||Find tissues and cell lines supported by RNA-seq analysis to express PRKACA.
|Nucleotide Accession #
||This antibody may replace item sc-111700 from Santa Cruz Biotechnology.
||sc-111700; sc-28315; sc-28316; sc-28892; sc-30666; sc-30668; sc-30669; sc-36237; sc-36240; sc-36241; sc-365615; sc-390548; sc-48412; sc-514087; sc-515038; sc-515039; sc-903
||Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish
|Predicted Homology Based on Immunogen Sequence
||Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 93%; Sheep: 100%; Zebrafish: 86%
|Human Muscle ||WB Suggested Anti-PRKACA Antibody Titration: 0.2-1 ug/ml|
Positive Control: Human Muscle
|Human heart||Rabbit Anti-PRKACA Antibody|
Catalog Number: ARP57828_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Exposure Time: 0.5 - 2.0 sec