UBA3 Antibody - N-terminal region (ARP50493_P050)

Data Sheet
 
Product Number ARP50493_P050
Product Page www.avivasysbio.com/uba3-antibody-n-terminal-region-arp50493-p050.html
Name UBA3 Antibody - N-terminal region (ARP50493_P050)
Protein Size (# AA) 463 amino acids
Molecular Weight 52kDa
NCBI Gene Id 9039
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Ubiquitin-like modifier activating enzyme 3
Alias Symbols NAE2, UBE1C, hUBA3
Peptide Sequence Synthetic peptide located within the following region: EGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCEL
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Li,T., (2006) Arch. Biochem. Biophys. 453 (1), 70-74
Description of Target UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Protein Interactions UBA3; UBC; IMPA2; CPNE2; NEDD8; CAMK1D; NSFL1C; DCPS; KDM1A; PAPSS1; NAE1; XPO1; PCYT2; CAPN2; AHCY; ACAT2; NT5DC1; NUDCD2; TXNDC17; WDR61; UFC1; DSTN; VCL; UBE2H; PRDX2; TWF1; UBE2M; UIMC1; CHD3;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-UBA3 (ARP50493_P050) antibody
Blocking Peptide For anti-UBA3 (ARP50493_P050) antibody is Catalog # AAP50493 (Previous Catalog # AAPP29786)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human UBA3
Uniprot ID Q8TBC4
Protein Name NEDD8-activating enzyme E1 catalytic subunit
Protein Accession # NP_003959
Purification Affinity Purified
Nucleotide Accession # NM_003968
Tested Species Reactivity Human
Gene Symbol UBA3
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
Image 1
Human NT-2
Sample Type: 1. Human NT-2 cells (60ug)2. mouse brain extracts (80ug)
Primary antibody dilution: 2ug/ml
Secondary antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary antibody dilution: 1: 20,000
Image Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science _x005F_x000D_ _x005F_x000D_
Image 2
Human Brain
Sample Type:
Human brain stem cells
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
UBA3: Red DAPI:Blue
Gene Name:
UBA3
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science
Image 3
Human Pancreas
WB Suggested Anti-UBA3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Pancreas
 

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