Product Number |
ARP50493_P050 |
Product Page |
www.avivasysbio.com/uba3-antibody-n-terminal-region-arp50493-p050.html |
Name |
UBA3 Antibody - N-terminal region (ARP50493_P050) |
Protein Size (# AA) |
463 amino acids |
Molecular Weight |
52kDa |
NCBI Gene Id |
9039 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Ubiquitin-like modifier activating enzyme 3 |
Alias Symbols |
NAE2, UBE1C, hUBA3 |
Peptide Sequence |
Synthetic peptide located within the following region: EGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCEL |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Li,T., (2006) Arch. Biochem. Biophys. 453 (1), 70-74 |
Description of Target |
UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
Protein Interactions |
UBA3; UBC; IMPA2; CPNE2; NEDD8; CAMK1D; NSFL1C; DCPS; KDM1A; PAPSS1; NAE1; XPO1; PCYT2; CAPN2; AHCY; ACAT2; NT5DC1; NUDCD2; TXNDC17; WDR61; UFC1; DSTN; VCL; UBE2H; PRDX2; TWF1; UBE2M; UIMC1; CHD3; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-UBA3 (ARP50493_P050) antibody |
Blocking Peptide |
For anti-UBA3 (ARP50493_P050) antibody is Catalog # AAP50493 (Previous Catalog # AAPP29786) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human UBA3 |
Uniprot ID |
Q8TBC4 |
Protein Name |
NEDD8-activating enzyme E1 catalytic subunit |
Protein Accession # |
NP_003959 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_003968 |
Tested Species Reactivity |
Human |
Gene Symbol |
UBA3 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86% |
Image 1 | Human NT-2
| Sample Type: 1. Human NT-2 cells (60ug)2. mouse brain extracts (80ug) Primary antibody dilution: 2ug/ml Secondary antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary antibody dilution: 1: 20,000 Image Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science _x005F_x000D_ _x005F_x000D_ |
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Image 2 | Human Brain
| Sample Type: Human brain stem cellsPrimary Antibody Dilution: 1:500Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution: 1:1000Color/Signal Descriptions: UBA3: Red DAPI:BlueGene Name: UBA3Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science |
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Image 3 | Human Pancreas
| WB Suggested Anti-UBA3 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: Human Pancreas |
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