CENPA Antibody - middle region (ARP46041_P050)

• Product Number: ARP46041_P050
• Name: CENPA Antibody - middle region (ARP46041_P050)
• Protein Size (# AA): 140 amino acids
• Molecular Weight: 16kDa
• NCBI Gene Id: 1058
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Centromere protein A
• Alias Symbols: CenH3, CENP-A
• Peptide Sequence: Synthetic peptide located within the following region: ALQEAAEAFLVHLFEDAYLLTLHAGRVTLFPKDVQLARRIRGLEEGLG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Orthaus,S., (2008) Chembiochem 9 (1), 77-92
• Description of Target: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA is a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Protein Interactions: HIST1H4A; HIST1H2AB; H3F3A; HIST1H2BB; DIAPH3; DIAPH1; NPM1; HIST1H1A; H2AFZ; H2AFX; CENPC; CENPB; HIST3H2BB; HIST4H4; CENPT; CENPU; CENPM; CENPH; CENPN; HJURP; SUPT16H; H2AFY; AURKB; HIST1H3E; HIST2H2AC; SSRP1; RUVBL1; XRCC5; PRKDC; HNRNPU; EIF4A1; DDX5;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-CENPA (ARP46041_P050) antibody is Catalog # AAP46041 (Previous Catalog # AAPP26882)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CENPA
• Uniprot ID: P49450
• Protein Name: Histone H3-like centromeric protein A
• Sample Type Confirmation:

  CENPA is strongly supported by BioGPS gene expression data to be expressed in 721_B, HeLa

• Protein Accession #: NP_001800
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001809
• Tested Species Reactivity: Human
• Gene Symbol: CENPA
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 77%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 100%; Zebrafish: 85%
• Image 1: Human 721_B
  
  Host: Rabbit
  Target Name: CENPA
  Sample Type: 721_B
  Antibody Dilution: 1.0ug/mlCENPA is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
• Image 2: Human HeLa
  
  WB Suggested Anti-CENPA Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: Hela cell lysate.CENPA is strongly supported by BioGPS gene expression data to be expressed in HeLa