| Product Number |
ARP45827_T100 |
| Product Page |
www.avivasysbio.com/chaf1b-antibody-c-terminal-region-arp45827-t100.html |
| Name |
CHAF1B Antibody - C-terminal region (ARP45827_T100) |
| Protein Size (# AA) |
559 amino acids |
| Molecular Weight |
61kDa |
| Subunit |
B |
| NCBI Gene Id |
8208 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Chromatin assembly factor 1, subunit B (p60) |
| Alias Symbols |
CAF1, MPP7, CAF-1, CAF1A, CAF1P60, CAF-IP60, MPHOSPH7 |
| Peptide Sequence |
Synthetic peptide located within the following region: PPSSVPTSVISTPSTEEIQSETPGDAQGSPPELKRPRLDENKGGTESLDP |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Beausoleil,S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135 |
| Description of Target |
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. CHAF1B corresponds to the p60 subunit and is required for chromatin assembly after replication. CHAF1B is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. |
| Protein Interactions |
UBC; SOX2; CNOT6L; env; CBX6; CBX5; HIST1H3B; HIST1H3A; CHAF1A; PCNA; APP; HIST2H3C; HIST1H4F; HIST1H3E; HIST3H3; SUMO2; BANF1; ASF1B; ASF1A; SETDB1; RBBP4; CDK2; CDK1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-CHAF1B (ARP45827_T100) antibody |
| Additional Information |
IHC Information: Lane A: Marker. Lane B: Jurkat cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%. |
| Blocking Peptide |
For anti-CHAF1B (ARP45827_T100) antibody is Catalog # AAP45827 (Previous Catalog # AAPP26764) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human CHAF1B |
| Uniprot ID |
Q13112 |
| Protein Name |
Chromatin assembly factor 1 subunit B |
| Sample Type Confirmation |
CHAF1B is supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_005432 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_005441 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
CHAF1B |
| Predicted Species Reactivity |
Human |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Human: 100% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-CHAF1B Antibody Titration: 5.0ug/ml
Positive Control: Jurkat cell lysateCHAF1B is supported by BioGPS gene expression data to be expressed in Jurkat |
|
| Image 2 | Human kidney
 | Human kidney |
|
