CBS Antibody - N-terminal region (ARP45746_T100)

Data Sheet
 
Product Number ARP45746_T100
Product Page www.avivasysbio.com/cbs-antibody-n-terminal-region-arp45746-t100.html
Name CBS Antibody - N-terminal region (ARP45746_T100)
Protein Size (# AA) 551 amino acids
Molecular Weight 61 kDa
NCBI Gene Id 875
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Cystathionine-beta-synthase
Alias Symbols CBSL, HIP4
Peptide Sequence Synthetic peptide located within the following region: RCIIVMPEKMSSEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNE
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Urreizti,R., (2006) J. Hum. Genet. 51 (4), 305-313
Description of Target CBS is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.The protein encoded by this gene is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.
Protein Interactions UBASH3A; UBE2I; PSMA1; PRKAG1; PIN1; EHHADH; CBS; SUMO1; UBC; NEDD8; PIAS3; RANBP9; PIAS1; CBX4; SRP14; RAD23B; PKM; NUCB1; IREB2; HSF1; LRSAM1; C14orf142; UBA5; IPO4; GOPC; PARP6; NPLOC4; DERA; ERO1L; DBNL; GPN1; PDLIM5; PLIN3; UBA2; GPSM1; COL4A3BP; UGP
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
WBY
SPR
YCHAROS
Datasheets/Manuals Printable datasheet for anti-CBS (ARP45746_T100) antibody
Additional Information IHC Information: Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.
Blocking Peptide For anti-CBS (ARP45746_T100) antibody is Catalog # AAP45746 (Previous Catalog # AAPP26703)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CBS
Uniprot ID P35520
Protein Name Cystathionine beta-synthase
Publications

Dahlhoff, C. et al. Hepatic methionine homeostasis is conserved in C57BL/6N mice on high-fat diet despite major changes in hepatic one-carbon metabolism. PLoS One 8, e57387 (2013). 23472083

Dahlhoff, C. et al. Methyl-donor supplementation in obese mice prevents the progression of NAFLD, activates AMPK and decreases acyl-carnitine levels. Mol. Metab. 3, 565-80 (2014). 25061561

Fernandes, V. S. et al. Endogenous hydrogen sulfide has a powerful role in inhibitory neurotransmission to the pig bladder neck. J. Urol. 189, 1567-73 (2013). 23063804

Geillinger, K. E. et al. Hepatic metabolite profiles in mice with a suboptimal selenium status. J. Nutr. Biochem. 25, 914-22 (2014). 24917045

Markand, S. et al. Cystathionine beta synthase expression in mouse retina. Curr. Eye Res. 38, 597-604 (2013). 23470016

Porteus, C. S. et al. The role of hydrogen sulphide in the control of breathing in hypoxic zebrafish (Danio rerio). J. Physiol. 592, 3075-88 (2014). 24756639

Spellman, C., Ahmed, M. M., Dubach, D. & Gardiner, K. J. Expression of trisomic proteins in Down syndrome model systems. Gene 512, 219-225 (2013). 23103828

Yamane, S., Kanno, T., Nakamura, H., Fujino, H. & Murayama, T. Hydrogen sulfide-mediated regulation of contractility in the mouse ileum with electrical stimulation: Roles of l-cysteine, cystathionine beta-synthase, and K(+) channels. Eur. J. Pharmacol. 740, 112-20 (2014). 25008073

Protein Accession # NP_000062
Purification Protein A purified
Nucleotide Accession # NM_000071
Tested Species Reactivity Human, Mouse, Rat
Gene Symbol CBS
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 86%; Zebrafish: 93%
Image 1
Mouse Brain
Mouse Brain
Image 2
Human Kidney
Kidney
Image 3
HepG2
Host: Rabbit
Target Name: CBS
Sample Type: HepG2
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 0.12
Image 4
Rat Liver
Host: Rabbit
Target Name: CBS
Sample Tissue: Rat Liver
Antibody Dilution: 1ug/ml
Image 5
Rat Liver
Host: Rat
Target Name: CBS
Sample Tissue: Rat Liver
Antibody Dilution: 1ug/ml
 

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