RORA Antibody - N-terminal region (ARP45606_T100)

• Product Number: ARP45606_T100
• Name: RORA Antibody - N-terminal region (ARP45606_T100)
• Protein Size (# AA): 548 amino acids
• Molecular Weight: 63 kDa
• NCBI Gene Id: 6095
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: RAR-related orphan receptor A
• Alias Symbols: ROR1, ROR2, ROR3, RZRA, NR1F1, IDDECA, RZR-ALPHA
• Peptide Sequence: Synthetic peptide located within the following region: CGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRC
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Boukhtouche,F., (2006) J. Neurochem. 96 (6), 1778-1789
• Description of Target: The protein encoded by RORA is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene.The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Four transcript variants encoding different isoforms have been described for this gene.
• Protein Interactions: GFAP; NSD1; NCOA1; PSMC5; SMARCD3; PTBP1; VPRBP; UBC; EZH2; DDB1; LMO3; NR0B1; ZXDC; PNRC2; COPS5; PNRC1; EIF3I; PROX1; LRIF1; RUVBL1; CEBPB; NCOA2; MED1; EP300; RORA; MYOD1; NME2; NME1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-RORA (ARP45606_T100) antibody is Catalog # AAP45606 (Previous Catalog # AAPP11885)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RORA
• Uniprot ID: P35398-3
• Protein Name: Nuclear receptor ROR-alpha
• Protein Accession #: NP_002934
• Purification: Protein A purified
• Nucleotide Accession #: NM_002943
• Tested Species Reactivity: Human
• Gene Symbol: RORA
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 86%; Zebrafish: 93%
• Image 1: Human HepG2
  
  WB Suggested Anti-RORA Antibody Titration: 1.25ug/ml
  Positive Control: HepG2 cell lysate
• Image 2: Western Blot
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.