NR3C2 Antibody - middle region (ARP45599_T100)

• Product Number: ARP45599_T100
• Name: NR3C2 Antibody - middle region (ARP45599_T100)
• Protein Size (# AA): 984 amino acids
• Molecular Weight: 107 kDa
• NCBI Gene Id: 4306
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Nuclear receptor subfamily 3, group C, member 2
• Alias Symbols: MR, MCR, MLR, NR3C2VIT
• Peptide Sequence: Synthetic peptide located within the following region: RRKNCPACRLQKCLQAGMNLGARKSKKLGKLKGIHEEQPQQQQPPPPPPP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Liu,G., (2006) Biochem. Biophys. Res. Commun. 342 (4), 1291-1296
• Description of Target: NR3C2 is a receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. It binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
• Protein Interactions: RABAC1; RXRA; HSP90AA1; ACSL1; UBC; SRC; PPP5C; PPID; FKBP5; FKBP4; STUB1; AIP; TRIM24; NRIP1; DNAJC7; CAND1; SUMO1; UBE2I; EIF3I; PROX1; PIAS1; PIAS3; HSPA4; PTGES3; AR; NR3C1; ACTA1; ACTG1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-NR3C2 (ARP45599_T100) antibody is Catalog # AAP45599 (Previous Catalog # AAPP11878)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NR3C2
• Uniprot ID: P08235
• Protein Name: Mineralocorticoid receptor
• Sample Type Confirmation:

  NR3C2 is supported by BioGPS gene expression data to be expressed in A172

• Protein Accession #: NP_000892
• Purification: Protein A purified
• Nucleotide Accession #: NM_000901
• Tested Species Reactivity: Human
• Gene Symbol: NR3C2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Pig, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 83%; Guinea Pig: 75%; Horse: 86%; Human: 100%; Mouse: 92%; Pig: 93%; Rabbit: 100%; Rat: 92%
• Image 1: Human kidney
  
  Human kidney
• Image 2: Human Ovary, Human lung
  
  Host: Rabbit
  Target: NR3C2
  Positive control (+): Human Ovary (OV)
  Negative control (-): Human lung (LU)
  Antibody concentration: 0.5ug/ml
• Image 3: Western Blot
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.