RPN2 Antibody - N-terminal region (ARP45400_P050)

Data Sheet
 
Product Number ARP45400_P050
Product Page www.avivasysbio.com/rpn2-antibody-n-terminal-region-arp45400-p050.html
Name RPN2 Antibody - N-terminal region (ARP45400_P050)
Protein Size (# AA) 631 amino acids
Molecular Weight 69kDa
Subunit 2
NCBI Gene Id 6185
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Ribophorin II
Alias Symbols SWP1, RPNII, RIBIIR, RPN-II
Peptide Sequence Synthetic peptide located within the following region: ASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVA
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Shibatani,T., (2005) Biochemistry 44 (16), 5982-5992
Description of Target RPN2 is a type I integral membrane protein found only in the rough endoplasmic reticulum. The protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains.This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1.
Protein Interactions UBC; FUS; FAF1; VAPB; EED; ADRB2; CLN3; MMS19; RCHY1; UBL4A; ESR1; RHOA; RPN1; ILF3; PDIA3; CANX; ATP5A1; STT3B; TM9SF2; FBXO6; CUL3; FBXO25; SIRT7; TCF3; AUP1; ARRB2; NDRG1; SUMO2; FBN3; RBBP4; NGFR; HDAC5; MYC; RNF185; H2AFX; BRE; ADRM1; EZH2; GSTK1; TM
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-RPN2 (ARP45400_P050) antibody
Additional Information IHC Information: HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.
Blocking Peptide For anti-RPN2 (ARP45400_P050) antibody is Catalog # AAP45400 (Previous Catalog # AAPP26368)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human RPN2
Uniprot ID P04844
Protein Name Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
Publications

Expression of ribophorine II is a promising prognostic factor in human gastric adenocarcinoma. Int. J. Oncol. 50, 448-456 (2017). 28035352

Sample Type Confirmation

RPN2 is strongly supported by BioGPS gene expression data to be expressed in HepG2

Protein Accession # NP_002942
Purification Affinity Purified
Nucleotide Accession # NM_002951
Tested Species Reactivity Human
Gene Symbol RPN2
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 93%; Rabbit: 100%; Rat: 100%
Image 1
Human Fetal Liver
Host: Rabbit
Target Name: RPN2
Sample Tissue: Human Fetal Liver
Antibody Dilution: 1.0ug/ml
Image 2
Human Skin
Human Skin
Image 3
Human Bronchial Epithelial Tissue
Rabbit Anti-RPN2 Antibody
Catalog Number: ARP45400_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

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