RNF128 Antibody - C-terminal region (ARP43311_T100)

• Product Number: ARP43311_T100
• Name: RNF128 Antibody - C-terminal region (ARP43311_T100)
• Protein Size (# AA): 428 amino acids
• Molecular Weight: 47 kDa
• NCBI Gene Id: 79589
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Ring finger protein 128, E3 ubiquitin protein ligase
• Alias Symbols: GRAIL
• Peptide Sequence: Synthetic peptide located within the following region: LEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDETPNQETAVREI
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Soares,L., (2004) Nat. Immunol. 5 (1), 45-54
• Description of Target: RNF128 is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of RNF128 in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of RNF128 was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype.The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Protein Interactions: CEP76; APPBP2; TP53; UBE2H; UBE2E1; UBE2D1; Arhgdia; Arhgdib; RNF128; UBE2T; UBE2I; UBE2N; CD40LG; OTUB1; USP8;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-RNF128 (ARP43311_T100) antibody is Catalog # AAP43311 (Previous Catalog # AAPP11386)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RNF128
• Uniprot ID: Q8TEB7
• Protein Name: E3 ubiquitin-protein ligase RNF128
• Sample Type Confirmation:

  RNF128 is supported by BioGPS gene expression data to be expressed in HepG2

• Protein Accession #: NP_919445
• Purification: Protein A purified
• Nucleotide Accession #: NM_194463
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: RNF128
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%
• Image 1: Mouse CD4 T
  
  Lanes:
  1: CD4 T cell lysate from WT mice, 2: CD4 T cell lysate from WT mice treated with anti-CD3/CD28, 3: CD4 T cell lysate from WT mice treated with FK506, 4: CD4 T cell lysate from WT mice treated with anti-CD3/CD28 and FK506, 5: CD4 T cell lysate from TRPV1 KO mice, 6: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28, 7: CD4 T cell lysate from TRPV1 KO mice treated with FK506, 8: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28 and FK506.
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit HRP
  Secondary Antibody Dilution:
  1:10,000
  Gene Name:
  RNF128
  Submitted by:
  Christine Quinley, UCSD
  
• Image 2: Human HepG2
  
  WB Suggested Anti-RNF128 Antibody Titration: 1.25ug/ml
  Positive Control: HepG2 cell lysateRNF128 is supported by BioGPS gene expression data to be expressed in HepG2
• Image 3:
  
  25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.