| Product Number |
ARP43158_T100 |
| Product Page |
www.avivasysbio.com/prpf19-antibody-middle-region-arp43158-t100.html |
| Name |
PRPF19 Antibody - middle region (ARP43158_T100) |
| Protein Size (# AA) |
504 amino acids |
| Molecular Weight |
55kDa |
| NCBI Gene Id |
27339 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) |
| Alias Symbols |
PSO4, SNEV, PRP19, UBOX4, hPSO4, NMP200 |
| Peptide Sequence |
Synthetic peptide located within the following region: PSVVGAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVK |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Voglauer,R., (2006) Exp. Cell Res. 312 (6), 746-759 |
| Description of Target |
PRPF19 plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. It binds double-stranded DNA in a sequence-nonspecific manner. PRPF19 acts as a structural component of the nuclear framework. It may also serve as a support for spliceosome binding and activity. It is essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. It also may have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.In S. cerevisiae, Pso4 has pleiotropic functions in DNA recombination and in error-prone nonhomologous end-joining DNA repair.[supplied by OMIM]. |
| Protein Interactions |
HUWE1; USP4; UBE2D3; SUMO2; CEP76; CEP250; TUBGCP3; CEP57; VCP; UBC; SUMO1; NEDD8; LIN28A; EXOC7; EXOC3; RPA3; RPA2; RPA1; RNF2; SUZ12; EED; rev; MEPCE; SEC13; PARK2; TARDBP; UBD; CSNK2A2; UCHL5; WHSC1; U2AF1; PRCC; HSP90AA1; HSPB1; FN1; RBM10; vif; CDC40 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PRPF19 (ARP43158_T100) antibody |
| Blocking Peptide |
For anti-PRPF19 (ARP43158_T100) antibody is Catalog # AAP43158 (Previous Catalog # AAPP25128) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human PRPF19 |
| Uniprot ID |
Q9UMS4 |
| Protein Name |
Pre-mRNA-processing factor 19 |
| Publications |
Song, G., Cui, Y., Zhong, N. & Han, J. Proteomic characterisation of pancreatic islet beta-cells stimulated with pancreatic carcinoma cell conditioned medium. J. Clin. Pathol. 62, 802-7 (2009). 19403515 |
| Protein Accession # |
NP_055317 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_014502 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
PRPF19 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-PRPF19 Antibody Titration: 2.5ug/ml
Positive Control: Jurkat cell lysate |
|
| Image 2 | mouse brain
 | WB Suggested Anti-PRPF19 Antibody
Positive Control: Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus
Primary Antibody Dilution : 1:1000
Secondary Antibody : Anti rabbit - IR-dye
Secondry Antibody Dilution : 1:10,000
Submitted by: Anonymous |
|
| Image 3 | Human kidney
 | Human kidney |
|
