UBE2M Antibody - N-terminal region (ARP43030_P050)

• Product Number: ARP43030_P050
• Name: UBE2M Antibody - N-terminal region (ARP43030_P050)
• Protein Size (# AA): 183 amino acids
• Molecular Weight: 21kDa
• NCBI Gene Id: 9040
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Ubiquitin-conjugating enzyme E2M
• Alias Symbols: UBC12, hUbc12, UBC-RS2
• Peptide Sequence: Synthetic peptide located within the following region: IKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISF
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007)
• Description of Target: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. It is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
• Protein Interactions: DCUN1D1; DCUN1D4; RBX1; UBC; MARCH7; UBE2M; CUL1; DCUN1D3; SMURF1; CAND1; DCUN1D2; NEDD8; DCUN1D5; LMNA; VCAM1; ITGA4; FN1; VCP; UBE2D2; TYMS; TST; VAMP2; PARK2; PINK1; UBE2T; SEC22B; SLC9A3R2; APP; CUL3; CUL4A; TP53; UBD; COPS6; UBA3; NAE1; CUL2; Nedd4;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-UBE2M (ARP43030_P050) antibody is Catalog # AAP43030 (Previous Catalog # AAPP11237)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2M
• Uniprot ID: P61081
• Protein Name: NEDD8-conjugating enzyme Ubc12
• Sample Type Confirmation:

  UBE2M is supported by BioGPS gene expression data to be expressed in 721_B, MCF7

• Protein Accession #: NP_003960
• Purification: Affinity Purified
• Nucleotide Accession #: NM_003969
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: UBE2M
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human 721_B
  
  WB Suggested Anti-UBE2M Antibody Titration: 0.2-1 ug/ml
  Positive Control: 721_B cell lysateUBE2M is supported by BioGPS gene expression data to be expressed in 721_B
• Image 2: Human MCF7
  
  Host: Rabbit
  Target Name: UBE2M
  Sample Type: Human MCF7
  Antibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in MCF7
• Image 3: Human 721_B
  
  Host: Rabbit
  Target Name: UBE2M
  Sample Type: Human 721_B
  Antibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in 721_B
• Image 4: Mouse Brain
  
  Sample Type:2. mouse brain extracts (80ug)
  Primary Antibody Dilution: 2ug/ml
  Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
  Secondary Antibody Dilution: 1: 20,000
  Image Submitted by: Yuzhi Chen
  University of Arkansas for Medical Science 
• Image 5: Mouse Brain
  
  Sample Type: NT2 cells
  Red: Antibody
  Blue: DAPI
  Primary Dilution: 1ug/50ul antibody
  Secondary Antibody: Alexa goat anti-rabbit 594
  Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences  
• Image 6: Human brain stem
  
  Sample Type:
  Human brain stem cells
  Primary Antibody Dilution:
  1:500
  Secondary Antibody:
  Goat anti-rabbit Alexa-Fluor 594
  Secondary Antibody Dilution:
  1:1000
  Color/Signal Descriptions:
  UBE2M: Red DAPI:Blue
  Gene Name:
  UBE2M
  Submitted by:
  Dr. Yuzhi Chen, University of Arkansas for Medical Science