UBE2L3 Antibody - C-terminal region (ARP43025_T100)

• Product Number: ARP43025_T100
• Name: UBE2L3 Antibody - C-terminal region (ARP43025_T100)
• Protein Size (# AA): 668 amino acids
• Molecular Weight: 75kDa
• NCBI Gene Id: 7332
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Ubiquitin-conjugating enzyme E2L 3
• Alias Symbols: E2-F1, L-UBC, UBCH7, UbcM4
• Peptide Sequence: Synthetic peptide located within the following region: TDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEK
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Yao,Y.L. (2003) J. Biol. Chem. 278 (43), 42560-42568
• Description of Target: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacteylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis.
• Protein Interactions: UBC; TRAF6; NEDD4; ITCH; MUL1; SMURF2; SMURF1; WWP2; WWP1; ARIH2; RNF144A; UBA1; PARK2; RNF144B; HACE1; ARIH1; UBE3A; UBE2L3; UBA6; UBR5; CACNA1B; MAP1LC3B; BAG3; HSPA4; AICDA; CCNB1IP1; FN1; RBX1; LIG4; ZPR1; USP9X; SF1; VDAC2; UCHL3; UBE2V2; UBE2D2; UBE
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-UBE2L3 (ARP43025_T100) antibody is Catalog # AAP43599 (Previous Catalog # AAPS13802)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human UBE2L3
• Uniprot ID: P68036
• Protein Name: Ubiquitin-conjugating enzyme E2 L3
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that UBE2L3 is expressed in Jurkat

• Protein Accession #: NP_003338
• Purification: Protein A purified
• Nucleotide Accession #: NM_004739
• Tested Species Reactivity: Human
• Gene Symbol: UBE2L3
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human Jurkat
  
  WB Suggested Anti-UBE2L3 Antibody Titration: 1.25ug/ml
  Positive Control: Jurkat cell lysateThere is BioGPS gene expression data showing that UBE2L3 is expressed in Jurkat
• Image 2: Human Intestine
  
  Human Intestine