Product Number |
ARP43006_T100 |
Product Page |
www.avivasysbio.com/ube2d1-antibody-middle-region-arp43006-t100.html |
Name |
UBE2D1 Antibody - middle region (ARP43006_T100) |
Protein Size (# AA) |
147 amino acids |
Molecular Weight |
16kDa |
NCBI Gene Id |
7321 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Ubiquitin-conjugating enzyme E2D 1 |
Alias Symbols |
SFT, UBCH5, UBC4/5, UBCH5A, E2(17)KB1 |
Peptide Sequence |
Synthetic peptide located within the following region: SQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHARE |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Description of Target |
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. |
Protein Interactions |
RBX1; STUB1; RNF7; RNF14; MID1; MDM2; EP300; CREBBP; ANAPC11; DTL; RNF115; MKRN2; NEDD4L; FAF2; TRIM32; MID2; RNF111; RNF5; RSP5; GPB1; NHLRC1; RFFL; UHRF2; DTX2; ZNRF1; TRIM63; RNF146; FBXO31; RNF26; TRIM39; RNF126; FBXW7; NEDD4; CUL1; CUL3; MKRN3; UBE3A |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-UBE2D1 (ARP43006_T100) antibody |
Blocking Peptide |
For anti-UBE2D1 (ARP43006_T100) antibody is Catalog # AAP42661 (Previous Catalog # AAPP24891) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human UBE2D1 |
Uniprot ID |
P51668 |
Protein Name |
Ubiquitin-conjugating enzyme E2 D1 |
Protein Accession # |
NP_003329 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_001204880 |
Tested Species Reactivity |
Human, Rat |
Gene Symbol |
UBE2D1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Pig, Rabbit, Zebrafish |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 79%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 93% |
Image 1 | Rat Skeletal Muscle
| Host: Rat Target Name: UBE2D1 Sample Tissue: Rat Skeletal Muscle Antibody Dilution: 1ug/ml |
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Image 2 | recombinant protein
| Lanes: 1: 40ng HIS-UBE2D1 protein 2: 40ng HIS-UBE2D2 protein 3: 40ng HIS-UBE2D3 protein 4: 40ng HIS-UBE2D4 protein 5: 40ng HIS-UBE2E1 protein 6: 40ng HIS-UBE2E2 protein 7: 40ng HIS-UBE2E3 protein 8: 40ng HIS-UBE2K protein 9: 40ng HIS-UBE2L3 protein 10: 40ng HIS-UBE2N protein 11: 40ng HIS-UBE2V1 protein 12: 40ng HIS-UBE2V2 protein. Primary Antibody Dilution: 1:500 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:50,000 Gene Name: UBE2D1 Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.
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Image 3 | Human Jurkat
| WB Suggested Anti-UBE2D1 Antibody Titration: 1.25ug/ml Positive Control: Jurkat cell lysate |
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