ANK1 Antibody - C-terminal region (ARP42566_T100)

• Product Number: ARP42566_T100
• Name: ANK1 Antibody - C-terminal region (ARP42566_T100)
• Protein Size (# AA): 155 amino acids
• Molecular Weight: 18kDa
• NCBI Gene Id: 286
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Ankyrin 1, erythrocytic
• Description:
  View product citations for ANK1 Antibody - C-terminal region (ARP42566_T100) on CiteAb
• Alias Symbols: ANK, SPH1, SPH2
• Peptide Sequence: Synthetic peptide located within the following region: VVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Chang,T.L., (2004) Cell. Mol. Biol. (Noisy-le-grand) 50 (1), 59-66
• Description of Target: Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known.Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.
• Protein Interactions: UBC; SPTA1; SUMO1; CD44; TGFBR1; SPTBN1; KCTD6; OBSCN; CUL3; NEDD8; SPTB; BLOC1S6; NFASC; NBPF3; TIAM1; L1CAM; TTN; SPTAN1; RHAG; EPB42; ITPR3; SLC4A1; SLC4A3;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-ANK1 (ARP42566_T100) antibody is Catalog # AAP42566 (Previous Catalog # AAPP24804)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ANK1
• Uniprot ID: Q53ER1
• Protein Name: Ankyrin 1 isoform 5 variant EMBL BAD97298.1
• Publications:

  Cytoplasmic gamma-actin and tropomodulin isoforms link to the sarcoplasmic reticulum in skeletal muscle fibers. J Cell Biol. 194, 105-20 (2011). 21727195

  Impaired muscle relaxation and mitochondrial fission associated with genetic ablation of cytoplasmic actin isoforms. FEBS J. 285, 481-500 (2018). 29265728

  Obscurin and KCTD6 regulate cullin-dependent small ankyrin-1 (sAnk1.5) protein turnover. Mol Biol Cell. 23, 2490-504 (2012). 22573887

  Obscurin determines the architecture of the longitudinal sarcoplasmic reticulum. J Cell Sci. 122, 2640-50 (2009). 19584095

• Protein Accession #: NP_065211
• Purification: Protein A purified
• Nucleotide Accession #: NM_020478
• Tested Species Reactivity: Human
• Gene Symbol: ANK1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human MCF7
  
  Host: Rabbit
  Target Name: ANK1
  Sample Tissue: Human MCF7
  Antibody Dilution: 1.0ug/ml
• Image 2: Human Lung
  
  Human Lung
• Image 3: Human Skin
  
  Human Skin