ACP1 Antibody - N-terminal region (ARP42178_T100)

• Product Number: ARP42178_T100
• Name: ACP1 Antibody - N-terminal region (ARP42178_T100)
• Protein Size (# AA): 158 amino acids
• Molecular Weight: 18 kDa
• NCBI Gene Id: 52
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Acid phosphatase 1, soluble
• Alias Symbols: HAAP, LMWPTP, LMW-PTP
• Peptide Sequence: Synthetic peptide located within the following region: PIAEAVFRKLVTDQNISENWRVDSAATSGYEIGNPPDYRGQSCMKRHGIP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Zabell,A.P., (2006) J. Biol. Chem. 281 (10), 6520-6527
• Description of Target: ACP1 belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate.The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene.
• Protein Interactions: EGFR; US3; VCAM1; ITGA4; FN1; BARD1; RPL12; UBC; COPS5; CDK2; MRPL20; FNBP1L; SFMBT1; EPHB2; EPHB1; FABP4; KDR; SPTAN1; ZAP70; EPHA2; LCK; INSR; FYN; CTNNB1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-ACP1 (ARP42178_T100) antibody is Catalog # AAP42178 (Previous Catalog # AAPP24603)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACP1
• Uniprot ID: P24666
• Protein Name: Low molecular weight phosphotyrosine protein phosphatase
• Sample Type Confirmation:

  ACP1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_004291
• Purification: Protein A purified
• Nucleotide Accession #: NM_004300
• Tested Species Reactivity: Human
• Gene Symbol: ACP1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 83%; Horse: 100%; Human: 100%; Mouse: 92%; Pig: 92%; Rabbit: 100%; Rat: 100%
• Image 1: human Kidney
  
  Rabbit Anti-ACP1 Antibody
  Catalog Number: ARP42178
  Paraffin Embedded Tissue: Human Kidney
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 2: Western Blot
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Recommended dilution for this antibody is 1-3 ug/mL. Canonical 18 kDa isoform is identified.