BAG2 Antibody - C-terminal region (ARP42177_T100)

• Product Number: ARP42177_T100
• Name: BAG2 Antibody - C-terminal region (ARP42177_T100)
• Protein Size (# AA): 211 amino acids
• Molecular Weight: 24 kDa
• NCBI Gene Id: 9532
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: BCL2-associated athanogene 2
• Alias Symbols: BAG-2, dJ417I1.2
• Peptide Sequence: Synthetic peptide located within the following region: VDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSK
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Arndt,V., (2005) Mol. Biol. Cell 16 (12), 5891-5900
• Description of Target: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
• Protein Interactions: BAG2; UBC; TP53; LGALS3BP; TUBGCP2; TUBG1; STIP1; TAB1; STUB1; DNAJB6; MLF2; TTC1; STXBP2; SGTA; DNAJB1; HSPA8; SHFM1; PINK1; RPA3; RPA2; RPA1; AHNAK; PARK2; LATS2; TARDBP; EGFR; BAG3; HDAC11; SOX2; NPM1; PAN2; WDR83; WDR77; CCAR2; METTL21B; DNAJC6; NOS2;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-BAG2 (ARP42177_T100) antibody is Catalog # AAP42177 (Previous Catalog # AAPP24602)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human BAG2
• Uniprot ID: O95816
• Protein Name: BAG family molecular chaperone regulator 2
• Publications:

  BAG2 promotes tumorigenesis through enhancing mutant p53 protein levels and function. Elife. 4, (2015). 26271008

• Sample Type Confirmation:

  BAG2 is supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_004273
• Purification: Protein A purified
• Nucleotide Accession #: NM_004282
• Tested Species Reactivity: Human
• Gene Symbol: BAG2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: siRUVBL transfected human Saos2
  
  Sample Type:
  Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human Saos2 cells Primary Antibody Dilution:
  1:1000 Secondary Antibody:
  Anti-rabbit-HRP Secondary Antibody Dilution:
  1:3000 Color/Signal Descriptions:
  BAG2 Gene Name:
  Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. Submitted by:
  
• Image 2: Human Kidney
  
  Rabbit Anti-BAG2 Antibody
  Catalog Number: ARP42177
  Paraffin Embedded Tissue: Human Kidney
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 3:
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.