DLAT Antibody - N-terminal region (ARP41994_T100)

• Product Number: ARP41994_T100
• Name: DLAT Antibody - N-terminal region (ARP41994_T100)
• Protein Size (# AA): 647 amino acids
• Molecular Weight: 69 kDa
• NCBI Gene Id: 1737
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Dihydrolipoamide S-acetyltransferase
• Alias Symbols: E2, PBC, DLTA, PDCE2, PDC-E2
• Peptide Sequence: Synthetic peptide located within the following region: WTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPTMQAGTIAR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Head,R.A., (2005) Ann. Neurol. 58 (2), 234-241
• Description of Target: DLAT is dihydrolipoamide acetyltransferase, the E2 subunit of the mammalian pyruvate dehydrogenase complex of the inner mitochondrial membrane. Patients with primary biliary cirrhosis show autoantibodies to DLAT.The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM].
• Protein Interactions: MDM2; UBC; ADRB2; PRKD3; PDHX; MMGT1; MRPL1; ACAD9; SYNCRIP; PDHA2; PDHA1; OGDH; NDUFS4; HSPA1L; DLD; COX5B; COPS5; CUL1; SIRT7; TSG101; KIAA0101; PSMA3; PDK4; PDK3; PDK2; PDK1; PDHB;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-DLAT (ARP41994_T100) antibody is Catalog # AAP41994 (Previous Catalog # AAPS11311)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DLAT
• Uniprot ID: P10515
• Protein Name: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
• Protein Accession #: NP_001922
• Purification: Protein A purified
• Nucleotide Accession #: NM_001931
• Tested Species Reactivity: Human
• Gene Symbol: DLAT
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
• Image 1: Human Jurkat
  
  Host: Rabbit
  Target Name: DLAT
  Sample Tissue: Human Jurkat
  Antibody Dilution: 1.0ug/ml
• Image 2: Human Kidney
  
  Rabbit Anti-DLAT Antibody
  Catalog Number: ARP41994
  Paraffin Embedded Tissue: Human Kidney
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 3: HepG2, Human liver
  
  Host: Rabbit
  Target: DLAT
  Positive control (+): HepG2 (HG)
  Negative control (-): Human liver (LI)
  Antibody concentration: 3ug/ml
• Image 4: Western Blot
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Isoforms are present from ~68 kDa to ~ 55 kDa.