UPF3B Antibody - N-terminal region (ARP40998_T100)

Data Sheet
 
Product Number ARP40998_T100
Product Page www.avivasysbio.com/upf3b-antibody-n-terminal-region-arp40998-t100.html
Name UPF3B Antibody - N-terminal region (ARP40998_T100)
Protein Size (# AA) 470 amino acids
Molecular Weight 52kDa
NCBI Gene Id 65109
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name UPF3 regulator of nonsense transcripts homolog B (yeast)
Description
Alias Symbols MRX62, MRX82, UPF3X, HUPF3B, MRXS14, RENT3B, UPF3BP1, UPF3BP2, UPF3BP3, Upf3p-X
Peptide Sequence Synthetic peptide located within the following region: MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEAL
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Lehner,B. (2004) Genome Res. 14 (7), 1315-1323
Description of Target UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctionsThis gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
Protein Interactions NCBP1; CIAO1; STAU1; UBC; UPF1; HECW2; RBM8A; UPF2; SMG1; EIF4A3; WIBG; WBP11; RNPS1; SART1; TOP2A; SNRPA1; NXF1; CAND1; COPS5; CUL5; USP21; EXOSC4; MCRS1; XRN1; TTC19; EIF6; UPF3B; HBB; UPF3A;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-UPF3B (ARP40998_T100) antibody
Blocking Peptide For anti-UPF3B (ARP40998_T100) antibody is Catalog # AAP40998 (Previous Catalog # AAPP22424)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human UPF3B
Uniprot ID Q9BZI7
Protein Name Regulator of nonsense transcripts 3B
Publications

Premature termination codon readthrough in human cells occurs in novel cytoplasmic foci and requires UPF proteins. J Cell Sci. 130, 3009-3022 (2017). 28743738

RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis. Sci Rep. 7, 41517 (2017). 28128343

Protein Accession # NP_075386
Purification Protein A purified
Nucleotide Accession # NM_023010
Tested Species Reactivity Human
Gene Symbol UPF3B
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Pig, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Human: 100%; Mouse: 93%; Pig: 93%; Rabbit: 93%; Rat: 100%
Image 1
Human Stomach
Rabbit Anti-UPF3B Antibody
Catalog Number: ARP40998
Paraffin Embedded Tissue: Human Stomach
Cellular Data: Epithelial cells of fundic gland
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Image 2
Human Jurkat
WB Suggested Anti-UPF3B Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate
 

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