Product Number |
ARP40998_T100 |
Product Page |
www.avivasysbio.com/upf3b-antibody-n-terminal-region-arp40998-t100.html |
Name |
UPF3B Antibody - N-terminal region (ARP40998_T100) |
Protein Size (# AA) |
470 amino acids |
Molecular Weight |
52kDa |
NCBI Gene Id |
65109 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
UPF3 regulator of nonsense transcripts homolog B (yeast) |
Description |
|
Alias Symbols |
MRX62, MRX82, UPF3X, HUPF3B, MRXS14, RENT3B, UPF3BP1, UPF3BP2, UPF3BP3, Upf3p-X |
Peptide Sequence |
Synthetic peptide located within the following region: MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEAL |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Lehner,B. (2004) Genome Res. 14 (7), 1315-1323 |
Description of Target |
UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctionsThis gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. |
Protein Interactions |
NCBP1; CIAO1; STAU1; UBC; UPF1; HECW2; RBM8A; UPF2; SMG1; EIF4A3; WIBG; WBP11; RNPS1; SART1; TOP2A; SNRPA1; NXF1; CAND1; COPS5; CUL5; USP21; EXOSC4; MCRS1; XRN1; TTC19; EIF6; UPF3B; HBB; UPF3A; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-UPF3B (ARP40998_T100) antibody |
Blocking Peptide |
For anti-UPF3B (ARP40998_T100) antibody is Catalog # AAP40998 (Previous Catalog # AAPP22424) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human UPF3B |
Uniprot ID |
Q9BZI7 |
Protein Name |
Regulator of nonsense transcripts 3B |
Publications |
Premature termination codon readthrough in human cells occurs in novel cytoplasmic foci and requires UPF proteins. J Cell Sci. 130, 3009-3022 (2017). 28743738
RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis. Sci Rep. 7, 41517 (2017). 28128343 |
Protein Accession # |
NP_075386 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_023010 |
Tested Species Reactivity |
Human |
Gene Symbol |
UPF3B |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Pig, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 93%; Pig: 93%; Rabbit: 93%; Rat: 100% |
Image 1 | Human Stomach
| Rabbit Anti-UPF3B Antibody Catalog Number: ARP40998 Paraffin Embedded Tissue: Human Stomach Cellular Data: Epithelial cells of fundic gland Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
|
Image 2 | Human Jurkat
| WB Suggested Anti-UPF3B Antibody Titration: 1.25ug/ml ELISA Titer: 1:312500 Positive Control: Jurkat cell lysate |
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