HNRPH3 Antibody - N-terminal region (ARP40721_T100)

• Product Number: ARP40721_T100
• Name: HNRPH3 Antibody - N-terminal region (ARP40721_T100)
• Protein Size (# AA): 346 amino acids
• Molecular Weight: 38 kDa
• NCBI Gene Id: 3189
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Heterogeneous nuclear ribonucleoprotein H3 (2H9)
• Alias Symbols: 2H9, HNRPH3
• Peptide Sequence: Synthetic peptide located within the following region: DYQGRSTGEAFVQFASKEIAENALGKHKERIGHRYIEIFRSSRSEIKGFY
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Andersen,J.S., (2002) Curr. Biol. 12 (1), 1-11
• Description of Target: HNRPH3 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein has two repeats of quasi-RRM domains that bind to RNAs. It is localized in nuclear bodies of the nucleus. This protein is involved in the splicing process and it also participates in early heat shock-induced splicing arrest by transiently leaving the hnRNP complexes.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is localized in nuclear bodies of the nucleus. This protein is involved in the splicing process and it also participates in early heat shock-induced splicing arrest by transiently leaving the hnRNP complexes. Multiple alternative transcript variants seem to be present for this gene and some appear to have intronic regions in the mRNA. Presently, only two transcript variants are fully described.
• Protein Interactions: UBC; SUMO2; SUMO3; STAU1; SUMO1; NEDD8; WWOX; ERG; RNF2; EED; rev; PARK2; STK24; HNRNPH3; HNRNPH1; HNRNPF; HNRNPD; HNRNPA1; FN1; DDX5; ITGA4; IL7R; HNRNPU; C17orf85; tat; HCVgp1; HNRNPUL1; HNRNPA0; CHERP; DDX17; VCAM1; RBM4; VCP; PUF60; SF3A3; HNRNPR; HNR
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-HNRNPH3 (ARP40721_T100) antibody is Catalog # AAP40721 (Previous Catalog # AAPY00973)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HNRPH3
• Uniprot ID: P31942
• Protein Name: Heterogeneous nuclear ribonucleoprotein H3
• Publications:

  Larriba, M. J. et al. Novel snail1 target proteins in human colon cancer identified by proteomic analysis. PLoS One 5, e10221 (2010). 20421926

• Sample Type Confirmation:

  HNRNPH3 is strongly supported by BioGPS gene expression data to be expressed in Raji

• Protein Accession #: NP_036339
• Purification: Protein A purified
• Nucleotide Accession #: NM_012207
• Tested Species Reactivity: Human
• Gene Symbol: HNRNPH3
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Image 1: Human Lung
  
  Rabbit Anti-HNRNPH3 Antibody
  Catalog Number: ARP40721
  Paraffin Embedded Tissue: Human alveolar cell
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 2: Human Kidney
  
  Rabbit Anti-HNRPH3 Antibody
  Catalog Number: ARP40721
  Paraffin Embedded Tissue: Human Kidney
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 3: Western Blot
  
  25 ug of the indicated Human cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Recommended dilution for this antibody is 1-3 ug/mL. Two closely sized isoforms are observed in some samples, and a smaller isoform is observed in 293T cells.