| Product Number |
ARP40649_P050 |
| Product Page |
www.avivasysbio.com/prpf8-antibody-n-terminal-region-arp40649-p050.html |
| Name |
PRPF8 Antibody - N-terminal region (ARP40649_P050) |
| Protein Size (# AA) |
2335 amino acids |
| Molecular Weight |
273kDa |
| NCBI Gene Id |
10594 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) |
| Alias Symbols |
PRP8, RP13, HPRP8, PRPC8, SNRNP220 |
| Peptide Sequence |
Synthetic peptide located within the following region: SHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPR |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Pena,V., (2007) Mol. Cell 25 (4), 615-624 |
| Description of Target |
Pre-mRNA splicing occurs in 2 sequential transesterification steps. PRPF8 is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. The gene encoding PRPF8 is a candidate gene for autosomal dominant retinitis pigmentosa.Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Protein Interactions |
SNRNP200; PRPF8; EFTUD2; UBC; CDKN1A; SUMO2; SUMO3; STAU1; LGR4; NEDD8; ERG; ASB15; WWOX; RPA3; RPA2; RPA1; EED; RNF2; SUZ12; rev; PIN1; NEDD4; FBXO6; PRPF4B; UBD; IGSF8; ICAM1; SND1; SF3B2; SLU7; ZNF830; WDR83; PRPF19; GPKOW; FN1; BCAS2; SF3B4; RBM5; VCA |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PRPF8 (ARP40649_P050) antibody |
| Blocking Peptide |
For anti-PRPF8 (ARP40649_P050) antibody is Catalog # AAP40649 (Previous Catalog # AAPY00904) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human PRPF8 |
| Uniprot ID |
Q6P2Q9 |
| Protein Name |
Pre-mRNA-processing-splicing factor 8 |
| Sample Type Confirmation |
PRPF8 is supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_006436 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_006445 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
PRPF8 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-PRPF8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysatePRPF8 is supported by BioGPS gene expression data to be expressed in Jurkat |
|
| Image 2 | Human A549 Whole Cell
 | Host: Rabbit
Target Name: PRPF8
Sample Tissue: Human A549 Whole Cell
Antibody Dilution: 3ug/ml |
|
