NXF1 Antibody - N-terminal region (ARP40639_T100)

• Product Number: ARP40639_T100
• Name: NXF1 Antibody - N-terminal region (ARP40639_T100)
• Protein Size (# AA): 619 amino acids
• Molecular Weight: 68kDa
• NCBI Gene Id: 10482
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Nuclear RNA export factor 1
• Alias Symbols: TAP, MEX67
• Peptide Sequence: Synthetic peptide located within the following region: MADEGKSYSEHDDERVNFPQRKKKGRGPFRWKYGEGNRRSGRGGSGIRSS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Chen,I.H., (2005) J. Virol. 79 (7), 3949-3961
• Description of Target: NXF1 is one member of a family of nuclear RNA export factor. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. NXF1 shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.
• Protein Interactions: KRT40; TIFA; GFAP; LDOC1; NUP62; MID2; TP53BP2; DVL3; SUMO2; UBC; SUZ12; EED; BMI1; TARDBP; NXT1; NUP98; RANBP2; ESRRA; KCTD5; TRA2A; SGTA; TRA2B; SRSF1; RAD51; ZBED2; CSNK2A1; EIF2A; ILF3; HNRNPC; HNRNPK; DHX15; DHX9; DDB1; DDX19A; LUC7L3; HP1BP3; RBMX;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-NXF1 (ARP40639_T100) antibody is Catalog # AAP40639 (Previous Catalog # AAPP10619)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NXF1
• Uniprot ID: Q9UBU9
• Protein Name: Nuclear RNA export factor 1
• Sample Type Confirmation:

  NXF1 is supported by BioGPS gene expression data to be expressed in HeLa, HepG2

• Protein Accession #: NP_006353
• Purification: Protein A purified
• Nucleotide Accession #: NM_006362
• Tested Species Reactivity: Human
• Gene Symbol: NXF1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 83%; Guinea Pig: 92%; Human: 100%; Mouse: 92%; Rat: 92%
• Image 1: Human kidney
  
  Rabbit Anti-NXF1Antibody
  Catalog Number: ARP40639
  Paraffin Embedded Tissue: Human Kidney
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 2: Human HepG2
  
  WB Suggested Anti-NXF1 Antibody Titration: 1.25ug/ml
  Positive Control: HepG2 cell lysateNXF1 is supported by BioGPS gene expression data to be expressed in HepG2
• Image 3: Human HeLa
  
  Lanes:
  Lane 1: 7ug HeLa lysate+EGFP SiRNA
  Lane 2: 7ug HeLa lysate+NXF1 SiRNA
  Primary Antibody Dilution:
  1:300
  Secondary Antibody:
  Anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:500
  Gene Name:
  NXF1
  Submitted by:
  Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University
  NXF1 is supported by BioGPS gene expression data to be expressed in HeLa